81 2018年6月
生化工程
过程工程学报
The Chinese Jourmal of Process Engineering
Vol.18 No.3 June2018
DOI: 10.12034/j.issn.1009-606X.217355
响应面法优化纤维素酶辅助从泡叶藻中提取泡叶藻聚糖的工艺
鲍青云，韦敬柳乙，
姜泽东1,2,3,4*
黄高凌1,234，
余刚，
肖安风12.34，倪辉12.34，
李清彪1234
朱艳冰1,234
(1.集美大学食品与生物工程学院，福建厦门361021：2.福建省食品微生物与酶工程重点实验室，福建厦门361021：
3.厦门南方海洋研究中心海藻资源化利用与深加工重点实验室，福建厦门361021：4.厦门市食品与生物工程技术研究中心，福建厦门361021) 摘要：通过单因素实验研究纤维素酶辅助提取泡叶藻聚糖时液料比、酶添加量、酶解温度、酶解时间等关键因素对泡叶藻聚糖提取率的影响，并进一步采用Box-Behnken实验设计和响应面分析法优化其工艺参数.结果表明，纤维素酶辅助提取泡叶藻聚糖的优化工艺条件为液料比30mL/g、酶浓度200IU/mL、酶解时间2.0h、酶解温度50℃，该条件下多糖提取率为
14.65%±0.73%，与模型预测值14.75%非常接近，采用响应面法对泡叶藻聚糖提取条件进行优化合理可行. 关键词：纤维素酶：辅助提取：泡叶藻聚糖；工艺优化：响应面法
中图分类号：TS201
文献标识码：A
文章编号：1009-606X(2018)03-0632-07
Optimization of Cellulase Assisted Extraction Conditions for Sulfated Polysaccharide (Ascophyllan)
fromAscophyllumnodosumbyResponseSurfaceMethodology
Qingyun BAOI,Jingliuyi WEI',Zedong JIANG12.3.4,GangYU',Gaoling HUANG 1234
, Anfeng XIAO1.234, Hui N1234, Qingbiao LI12.34
YanbingZHU1.2,3.4,
(I. College of Food and Biology Engineering, Jimei University, Xiamen, Fujian 361021, China;
2.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Technology, Xiamen, Fujian 361021, China;
3. Key Laboratory of Systemic Utilization and In-depth Processing Economic Seaweed,
Xiamen Southern Ocean Technology Center, Xiamen, Fujian 361021, China;
4. Research Center of Food and Biotechnology of Xiamen City, Xiamen, Fujian 361021, China)
Abstract: In order to obtain the optimum parameters for the cellulase assisted extraction of ascophyllan from brown seaweed Ascophyllum nodosum,the effects of several factors including water to biomass ratio, cellulase concentration, enzymolysis temperature, and enzymolysis time on the extraction yield of ascophyllan were investigated by using a single factor experiment and further optimized eeeaee ascophyllan extraction were ratio of water to biomass 30 mL/g, cellulase concentration 200 IU/mL, enzymolysis temperature 50 'C, enzymolysis time 2.0 h. Under these conditions, the actual extraction yield of ascophyllan was 14.65%±0.73%, in keeping approach with the predicted value by RSM (14.75%). Therefore, it is feasible that utilized RSM to optimize the conditions of extracting ascophyllan withthe assistofcellulase.
Key words: cellulase; assisted extraction; ascophyllan; optimum technology; response surface methodology
1
前言
泡叶藻(Ascophyllumnodosum)又称岩衣藻，是一种
大型海洋褐藻，与海带、裙带菜、马尾藻等褐藻相似，
含丰富的褐藻胶酸(GlginateAcid，约占藻体总糖的79%)：是工业生产褐藻胶、碘和甘露醇的主要原料之一[1,2],除褐藻胶酸外，泡叶藻含约占藻体总糖质13%的泡叶藻聚糖(Ascophyllan)和8%的岩藻聚糖(Fucoidan)[2),泡叶藻
收稿日期：201710-11，修回日期：20171123
基金项目：国家自然科学基金资助项目(编号：31501441)：集美大学学科建设基金资助项目(编号：ZC2014008)
作者简介；绝青云(1990-)，女，黑龙江省哈尔滨市人，硕士研究生，食品工程专业，Tel:13123395228,E-mail:qingyun_bao@126.com姜泽东，通讯联
系人，Tel: 18205990711,E-mail: zdjiang@jmu.edu.cn
引用格式：绝青云，韦敬柳乙，姜泽东，等，应面法优化纤维素酶辅助从泡叶藻中提取泡叶藻案糖的工艺，过程工程学报，2018，18(3）：632-638.
Bao QY, WeiJLY,JiangZD,etal.Optimization ofCellulaseAssisted Exraction Conditionsfor Sulfated Polysaccharide (Asophyllan)from
Ascophylm nodosum by Response Surface Methodology (in Chinese),. Chin.J. Process Eng.,2018, 18(3): 632638, DOI: 10.12034/j.issn.1009-606X.217355.
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