现代肿瘤医学2013年10月第21卷第10期
MODERN ONCOLOGY,Oct. 2013,VOL. 21,NO.10
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(编权：张志明）
香菇多糖联合多西他赛对胃癌BGC823细胞增殖的影响赵力"，李午生，李凯”，肖玉平2
Theeffectof lentinananddocetaxel ontheproliferationofBGC823cells Zhao Li', Li Wusheng',Li Kai',Xiao Yuping
'Department of Oncology,the Afiliated Shengjing Hospital of China Medical University, Liaoning Shenyang 110022, Chi-na;'Gastrointestinal Tumor Pathology Laboratory of Cancer Institute,Department of Tumor Pathology of General Surgery Institute,the First Hospital of China Medical University,Liaoning Shenyang 110001,China.
[Abstract]Objective : To investigate whether lentinan,lentinan combined with docetaxel can inhibit the cell prolif-eration in human gastric cancer cell line BCC823. Methods : BCC823 cells were divide into lentinan, docetaxel and lentinan combined docetaxel groups, Lentinan was added in the culturemedium to concentration of 1. 560μg/ml, 3. 125μg/ml,6.250μg/ml,12. 500μg/ml. Docetaxel was added in the culturemedium to concentration of 0. 625μg/ ml,1. 250μg/ml,2. 500μg/ml,5. 000μg/ml. MTT was applied to detect the proliferation of BGC823 cells. Apoptosis was detected by Annexin V/PI. Results: Lentinan could inhibit the proliferation of BGC823, Compared with the control group , the inhibitary rates were 67.7% ,56.0% ,42.1% and 23. 2% with different concentrations of lentin an( P <0. 05) . The inhibitory rates of the proliferation of BCC823 cells were significantly higher in combined with docetaxel than those in docetaxel alone group( P <0. 05). Lentinan can increase the apoptosis rates of BCC823 cells, the apoptosis rate was higher in combined with docetaxel group( P <0. 05). Conclusion: Lentinan can inhibit the pro-liferation of gastric cancer BGC823 cells,and can significantly enhance the inhibit rate of proliferation in docetaxel.[Key words]lentinan;docetaxel;BGC823;proliferation;apoptosis
Modem Oncology 2013,21(10) :2173 2176
【摘要】
自的：观察香菇多糖单药及联合多西他赛抑制胃瘤细胞BCC823增殖的作用。方法：分别用不同浓
度的香菇多糖、多西他赛和香菇多糖联合多西他赛处理胃癌细胞BGC823。香菇多糖终浓度分别为1.560μg/ ml,3.125μg/ml,6.250μg/ml,12.500μg/ml。多西他赛终浓度分别为0.625μg/ml，1.250μg/ml,2.500μg/ml， 5.000μg/ml。MTT法检测各组细胞增殖情况；同时用AnnexinV/PI法检测各组细胞的凋亡。结果：单药香菇多糖能抑制BCG823细胞的增殖，不同浓度（由高到低）对BCC823细胞的增殖抑制率分别为67.7%，56.0%、 42.1%和23.2%，与阴性对照组比较有统计学意义（P<0.05）。不同浓度香菇多糖联合多西他赛能增强多
【收稿日期】【修回日期】【作者单位】
【作者简介】
2013 03 15 2013 05 10
1中国医科大学附属盛京医院肿瘤1科，辽宁沈阳
110022
2中国医科大学附属第一医院肿瘤研究所胃肠肿瘤病理研究室，菩通外科研究所肿瘤病理研究室，辽宁沈阳
110001
赵力（1971-），女，河北任县人，讲师，主要从事消化道、乳腺恶性肿缩基磁及临床研究。E-mail：emu2hzhaoli@yahoo.cen