现代肿瘤医学2011年2月第19卷第2期
幽门螺杆菌L型感染对胃癌BGC-823细胞增殖的影响吴海波"，于东红，唐素兰"，承泽农2
· 229 ·
Investigation ofthereiationshipbetweenHelicobacterpyloriL-forminfectionandgastric carcinomacellsBGC-823proliferation
WU Hai - bo', YU Dong - hong", TANG Su lan", CHENG Ze - nong?
'Department of Pathology, The Affiliated Prorincial Hospital of Anhui Medical Unisersity, Hefei Anhui 230001, China; Department of Pathology, The First Afiliated Hospital of Bengbu Medical College, Anhai Key Laboratory of Infection and Immarnity, Bengbu Anhai 233004, China;'Department of Microbiology, Bengbu Medical College, Bengbu Anhui 233030, China.
Objective: To investigate the effect of Helicobacter pylori L form (Hp - L) on proliferation of gastric
[Abstract]
cancer cells BGC ~823 and the probable mechanism involved. Methods: BGC 823 cells were co incubated with different proportions of Hp L (1: 20,1: 100, 1: 500) and the experiments were carried out at different period of time, Morphology changes of BGC 823 cells were observed by inversion microscope; The proliferous rate of cell growth was assessed by MTT spectrophotometric analysis ; The cell cycle was measured by flow cytometry (FCM) ; Im munohistochemical staining was used to detect the expressions of Skp2, p53 and Ki 67 in BCC 823 cells. Results : By inversion microscope, BGC 823 cells were identified by their typical cobblestone configuration which adhibited the bottle wall with irregular morphology in normal situation, but when it were infected by Hp L, division cells in-creased and the cells grew bloom, many tumor giant cells could be seen, which indicated the growth acceleration of the cells; The growth curve of cell growth rate determined by MTT method showed that Hp - L could promote the BCC 823 cells proliferation; The FCM showed that Hp L changed the cell cycle distribution notably and caused a S phase advance , cells at G/G, phase were diminished; Expression of Skp2, p53 and Ki 67 show that the positive cells number gradually increased, all the results became more obviously with the increase of concentration of Hp L and the extention of time in a manner of concentration -- and time ~ dependent. Concision: Hp L may promote the proliferation of gastric cancer cells BGC 823 , its mechanism may be related to up regulation of Skp2 , p53 and Ki 67 expression.
[Key words] helicobacter pylori;L form; Skp2; Ki 67 ;p53 ; BGC 823 cells
Moderm Oncology 2011,19(02) :0229 0234
目的：研究密门螺杆菌L型(helicobacterpyloriL-fom，Hp-L型）感染对胃癌BCC-823细胞增殖
【摘要】
的影响，并操讨其作用机制。方法：将胃癌BGC-823细胞与Hp-L型以不同比例(1:20、1:100、1:500)共培养，以不加Hp-L型为对照组，在不同时段进行以下实验：倒置显微镜观察细跑形态学变化；四甲基壁唑氮蓝（MTT)法检测细胞生长增殖率；流式细胞仪（FCM)检测细胞凋亡率；免疫组化（SP法）检测癌基因（skp2）、抑痛基因（p53)和核增殖指数(Ki-67)的表达情况。结果：HpL型作用BGC-823细胞后，倒置显微镜观察到细胞分裂增多，增殖旺盛，瘤巨细胞增多，出现明显的生长加速现象；MTT法测定细胞生长曲线显示，Hp-L 型对胃癌BGC-823细跑增殖有促进作用;FCM检测可见，Hp-L型影响胃癌BGC-823细胞周期的分布，使
作用均呈细菌浓度和作用时间依赖性。结论：Hp-L型可促进胃癌BGC-823细胞增殖，其机制与上调Skp2、 p53和Ki-67蛋白的表达有关。
【关键调】幽门螺杆菌;L型;Skp2;Ki-67；p53；胃癌BCC-823细胞。
【收稿日期】【修日期】【基金项目】【作者单位】
【作者简介】【通讯作者】
201007 06 2010 10 27
安数省教育厅高校自然科学研究重点项目（编号：KJ2007A098），蚌埠市科技局2007年第一批科技项目（编号：11）
合肥230001
1安徽医科大学附属省立医院病理科，安徽
"蚌埠医学院第一附属医院病理科，安徽省感染与免疫重点实验室，安徽
蚌埠
233004
"蚌埠医学院微生物学教研室，安徽蚌埠233030
吴海波（1981-）男，安微合肥人，医师，主要从事肿瘤病理诊断和肿瘤发生发展的研究。R-mailbbruhaibo@sina.com 于东红（1955-），女，安徽蚌埠人，教授，硕士生导师，主要从事细荫L型与肿瘤关系的研究。