第37卷第2期 2017年6月
化学传感器 CHEMICALSENSORS
Vol. 37, No. 2 June.2017
结合分支杂交链式反应与增强拉曼的DNA传感器
张有1，夏莲，光进茂，任锐2
(1.曲阜师范大学化学与化工学院，山东省生命有机分析重点实验室，山东曲阜273165）(2.临沂大学化学化工学院，山东省肿瘤标志物检测重点实验室，山东临沂276000）
摘要：该研究建立了一种高灵缴度、高特异性的DNA检测方法，结合了分支杂交链式反应(B-HCR)与表面增强拉曼散射(SERS)两种技术，为实现这种结合，设计让靶DNA充当树枝状DNA大分子和SERS增强基底的衔接单元；基于经典的杂交链式反应而设计分支杂交链式反应（B-HCR)，还充当了树枝状DNA大分子和SERS的接头。绪合了B-HCR与SERS两种方式的优点，B-HCR便得在自组装中的每一轮生长都组装上更多的分支，从而实现拉曼活性基团的聚集；并结合SERS技术，增强放大信号，从而提高了灵敏度；而 DNA杂交反应的序列特异性保证了整个方法对于给定靶DNA的特异性。所报道的方法具备较高的灵敏度，还证明了足够的特异性来区分具有相似序列的DNA
关键调：DNA；分支杂交链式反应；表面增强拉曼散射；6"-羧基-x-罗丹明（ROX
DNA sensor that combined branchinghybridization chain reaction
andenhancedramanscattering Zhang You', Xia Lian', You Jin-mao', Ren Rui?
(1. Key Laboratory of LifeOrganic Analysis of Shandong Province, Qufu Normal University, Qufu 273165, China)(2. Shandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Chemistry and
Chemical Engineering, Linyi University, Linyi 276000, China)
Abstract: In this study, an assay for the detection of DNA of low abundance was established. The assay was con-structed by combining of Branching Hybridization Chain Reaction (BHCR) and SurfaceEnhanced Raman Scatter-ing (SERS). The target DNA was designed to serve as a linker between the BHCR and SERS. The advantages of the BHCR and SERS were combined. BHCR provides more branching in each round of growth of the assembly, thus leading to the mass aggregation of the Ramanactive tags; thus both amplification (through BHCR) and enhance-ment (through SERS) were applied on the signal, bringing about desirable sensitivity: also, specificity was provided, pe e sas a o are o nea oe o aadsaanas enor the triggering of the BHCR. Desirable sensitivity was achieved, and required specificity against DNA inputs with similar sequences was testified.
Key words: DNA; hybridization chain reaction; surface-enhanced raman scattering; 6carboxyxrhodamine
生等儿部分，其中靶标识别部分承担分析方法的
0引言
低含量DNA的检测，在生物化学研究、医学研究、病原体检测以及诊断中具有重大意义。这就要求开发简单、快速、高灵敏度和高选择性的 DNA分析方法。生物化学传感器中的DNA传感器可以分为靶标识别、信号转导以及放大信号产
特异性，信号转导和放大部分通常对微量靶标产生的微小信号进行放大处理，承担分析方法的灵敏度；而产生的信号将作为分析方法的读数输出。近年来，设计简单、易于操作的杂交链式反应(HCR)是新兴的信号转导和放大方法，因此得到了广泛关注和使用。
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