第30卷第3期 2010年9月
化学传感器 CHEMICALSENSORS
检测尿酸和葡萄糖电极式试纸条的研制
石平1，李建平2*，方成2，李玉平2(1.桂林优利特电子集团有限公司，广西桂林541001)(2.桂林理工大学化学与生物工程学院，广西桂林541004)
Vol. 30, No. 3 Sept.2010
摘要：根据双电极系统中铁氯化钾的电子媒介新模式原理研制一种电极式印制型尿酸和葡萄糖试条。试条由完全对等的丝网印制双碳电极组成，测定范围宽(-0.6~0.6V)，可根据检测物浓度和干扰情况选择测定电位和灵敏度。用尿酸和葡每糖试纸条测试35例病人血清样品中尿酸和葡葡糖的含量，并与医院常规分析
义(P>0.05)。铁氰化钾作为电子媒介体，其电子媒介作用是通过其转化成亚铁氯化钾来实现的，任何可导致上述转化发生的物质都可以借助铁氰化钾为电子媒介来测定，合理使用媒介体可以达到检测高过电位物质或放大检测信号等目的。
关键调：铁氰化钾；电子媒介；尿酸；帮糖
Thedesign of an electrochemical stripfor uricacidandglucoseassay
Shi Ping', Li Jian-ping’', Fang Cheng, Li Yu-ping
(1. Guilin medical Electronic Instrument Factory,Guilin 541001, China)
(2. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China)
Abstract: A disposal screenprintable uric acid and glucose electrode strip were fabricated based on the principle of ferricyanidemediated electron transfer as the electron mediator in twoelectrode system, Both strips exhibit ex-cellent linear responses to detecting substance in a wide measurement potential, 0.6 ~0.6 V, which be adjusted at will according to concentration of detecting substance and level of interference, Stuatistical analysis shows the result of detection by strip and conventional method have preferable relativity.Thirtyfive artificial samples of glucose or uric acid were taken and the contents of glucose and uric acid were tested using the proposed glucose and uric acid strips and the results were compared with that of routine analysis methods in hospital. It was evident that statistical analysis by Pairedsamples TTest showed the result of detection by the amperometric method gives the result of no significant differences (P was 0.427 and 0.079), Potassium ferricyanide acts as an electron mediator via the reductive reaction to potassium ferrocyanide, Consequently, the substance which could be oxidized by potassium fericyanide can be assayed by the amperometric method with potassium ferricyanide mediator. During the process, the analyte
with high overpotential could be determined and the current signal could be enhanced. Key words: potassium ferricyanide; electron mediator, uric acid; glucose
引言 0
生物传感器技术正被越来越多的应用在临床化学、生物医学的在线监测等[1-2]。这种新的检测手段和传统的分析方法相比具有分离和检测
基金项目：广西壮族自治区教育厅专利资助项目
*通讯联系人，E-mail:likianping@263.net 万方数据
一体、响应快、样品用量少，成本低等特点。目前商业化的生物试纸条已超过十余种13-7)，大致可分为光学型和电化学型两大类，前者可视为传统酶溶液比色法的衍生与移植，缺点是显色后颜色分布不均并且易受环境光线与温度影响18]，电化