2014年12月
化学传感器
SENSORS
CHEMICAL
Vol. 34, No. 4 Dec.2014
基于杂交链式反应放大信号的适体传感器的研究
彭德敏，衰若·
(西南大学化学化工学院，重庆400715)
要：该文基于多孔纳米钯复合物(pPdNPs)和杂交链式反应(HCR)对信号的放大，成功制备出一个高灵敏、无标记的夹心式新型电化学适体传感器用于凝血酶（TB)的检测。首先，沉积的纳米金有很好的生物相容性和大的比表面积，用于固载更多的凝血酶适体链(TBA)；同时，利用多孔纳米钯大的表面积来固载更多的
与SI、单链DNA(S,)和电活性物质亚甲基蓝(MB)的混合物通过杂交链式反应，形成内嵌有大量MB的双链 DNA(dsDNA)，以达到信号放大的效果。经实验研究表明，该传感器构建方法简单，切实可行，大大提高了电化学适体传感器的灵敏度。
关键词：电化学适体传感器；凝血酶；纳米粒子；杂交链式反应；多孔纳米钯
Anovelelectrochemicalaptasensorforthrombindetectionbasedon
hybridizationchainreactionasenhanced signals
PengDe-min，Yuan Ruo'
(School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China)
Abstract: Herein, we was prepared suecessfully a highsensitivity and unlabeled sandwichtype electrochemical aptasensor for thrombin (TB) detection based on porous NanoPalladium complexes (pPdNPs) and hybridization chain reaction (HCR). In the course of the experiment, Au nanoparticles electrodeposited (DpAu) had outstanding electrical conductivity to promote the electron transfer at the electrode interface and had good biocompatibility to load large amounts of thrombin binding aptamer (TBA). Moreover, pPdNPs was employed to load large number of TBAg and S,. In the present of TB, the TBAa pPdNPs S, was designed as the signal probe for the sandwiched aptasnesor, Then S, could trigger the HCR to lead to the formation of extended doublestranded DNA (dsDNA) polymers at the aid of S, and S, To achieve detection of thrombin, a mass of redox probe, methylene blue (MB), was intercalated into the dsDNA polymers through specific interaction. According to the linearity between TB sd ardaaaa aeenn adsaano strategy for the thrombin detection with high sensitivity and simple characteristics.
Key word: electrochemical aptasensor; thrombin; nanoparticle; hybridization chain reaction; porous Nano-Palladium complex(pPdNPs)
的许多特性。具有靶分子范围广、与配体作用亲
引言 0
适体也称为核酸适体、适配体、适配子等。可以是RNA，单链DNA或者双链DNA(1-3),长度一般为25~80个核苷酸[4-6)。适体与靶分子的结合和抗原一抗体作用相似，适体具有明显优于抗体
基金项目：国家自然科学基金资助项目(21275119,21105081)*通讯联系人，Tel.:023-68252277，E-mail:yuanruo@swu.edu.cn
万方数据
和高度稳定性、高特异性强、制备方法简单、安全经济等优点。目前，适体技术开始广泛应用于分子识别、实验诊断、疾病治疗、药物研究及生物传感器等方面。
由于可以实现对材料的修饰和优化以满足