.14·
2016 Vol.35 No.1 Serial No.287
China Brewing
Research Report
增效因子对木醋耗菌产细菌纤维素发酵液中物质变化的影响
贾青慧'卢红梅2*陈莉”张丽平
（00000000）
摘要在木醋杆菌(Acefobacterxylinum)产细菌纤维素(BC)培养基中添加一定量的增效因子,考察增效因子椰子水、玉米浆、 Tween-80、腰甲基纤维素(CMC)、烟酸和生物素对发酵液中细菌纤维素产量、总糖、总酸和有机酸含量的影响。结果表明10%玉米浆及60%椰子水对细菌纤维素产量的增效作用最强细菌纤维素产量分别为8.534g/L、6.008g/L与空白组相比分别增加了4.67、2.99
%0之中0 BC产量较为明显的增效作用有一定关系
关键词细菌纤维素总糖总酸有机酸增效因子
中图分类号.Q815
文章编号0254-5071(2016)01-0014-05
doi:10.11882/j.issn.02545071.2016.01.004
Effectof synergisticfactor on thesubstances changeoffermentation liquidwithbacterial
cellulose-producing Acetobacterxylinum JIA Qinghui', LU Hongmeil2*, CHEN Li, ZHANG Liping
(1.Schoo/ of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550000, China,
2.School of Liquor and Food Engineering, Guizhou University, Guiyang 550000 China)
Abstract: A certain amount of synergistic factors were added into the culture medium of bacterial cellulose-producing Acefobacter xylinum. The ef fect of coconut water, corn steep liquor, Tween-8o, CMC, niacin and biotin on bacterial cellulose (BC) yield and the content of total sugar, total acid and organic acid were investigated. The results showed that 10% corn steep liquor and 60% coconut water had the strongest effect on BC yield, and the BC yield were 8.534 g/L and 6.008 g/L, respectively. Compared with the blank group, it increased by 4.67 and 2.99 times respectively. Adding 6o% coconut water could reduce total sugar contents and promote A. xylinum synthesizing BC. After adding synergistic factors, the changes of total acid content were almost in the same, and appeared a decreasing trend. In the test group of 10% corn steep liquor, the organic acid content was the highest, and the proportion of glucose, lactic acid, acetic acid and butyl acid was the largest, which had a certain relationship with the effect of 10% corn steep liquor on BC yield.
Key words: bacterial cellulose; total sugar; total acid; organic acid; synergistic facto
细菌纤维素(bacterialcelluloseBC)是微生物产生的次级代谢产物在化学结构和化学组成上与植物纤维相司但与植物纤维的区别在于细菌纤维素存在形式单一、纯度高、不掺杂木质素或半纤维等其余多糖。BC以单一纤维形式存在且分子取向一致使其抗张强度、杨氏模量、硬度等高于植物纤维同时细菌纤维素的超细三维纳米纤维结构(直径在0.01～0.10μm)使其在伸长率、吸湿性、润胀度、柔软性和化学反应活性等方面优于植物纤维 BC还具有成膜性能好、膜抗撕能力强、保水量高、透气、透水性能好及湿强度高等特点,BC的诸多特异性使其可用在很多领域（如作为健康的纤维食品降低胆固醇叫作为优良的人工骨修复材料围用于生产薄形印刷纸提高印
收稿日期2015-09-11
刷性能围利用其超精细结构原位制备有机-无机杂化材料还可作为酶固定化载体间且细菌纤维素膜在质子交换膜燃料电池领域)具有美很好的应用前景()。目前国内外对于提高BC产量的研究主要集中在培育高产菌株、优化工艺条件、改进发酵方式和探讨代谢机理等方面国对于发酵过程中的物质变化较少研究但添加增效因子后各培养基内生长因子种类、含量存在差异，可能会影响木醋杆菌的代谢原有物质的消耗和新物质的生成也会不同，而这些不同与BC生成息息相关，
目前在我国BC主要通过自然发酵椰子水生产但由于原料具有季节性且自然发酵椰子水成分较为复杂严重限制了BC的商业化生产。在木醋杆菌产BC的试验中发
基金项目国家自然科学基金(31160338）贵州省科学技术基金(黔科合字[2010)2066)
作者简介贾青慧（1991-）女，士研究生研究方向为生物质资源化利用*通讯作者卢红梅(1967-）女教授博士研究方向为发酵工程以及食品科学
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