2012年3月，第18卷，第1期，117-124页 March2012，Vol.18，No.1，p.117124
高校地质学报
Geological Jourmal of China Universities
一株产铁载体细菌的筛选及其与云母的相互作用
何琳燕，张垠，盛下放，黄智
南京农业大学生命科学学院，农业部农业环境徽生物工程重点开放实验室，南京210095
摘要：产铁载体细菌与云母类矿物相互作用的研究有助于了解矿物生物风化和土壤形成的演化规律和机理。采用纯培养法自南京龙山废钾矿区酸模根际土壤分离筛选到一株高产荧光铁载体的细菌Z6，通过16SrDNA序列分析和生理生化反应将其鉴定为假单胞菌属（Pseudomonassp.）：通过室温静置培养试验研究Z6菌株与云母的相互作用结果表明，细菌生长代谢前期（8~15d）快速产生大量铁载体，菌株与矿物共培养75d后，产铁载体细菌对云母类矿物确实产生了风化作用，黑云母组发酵液中Fe含量增加了211倍，Si含量增加了约27.8倍，SEM显示云母表面出现溶蚀坑，细菌可以在矿物表面形成生物膜或
矿物-细菌复合物。不同矿物对共培养体系中细菌数量、荧光铁载体含量、溶液pH的影响不同。关键调：荧光铁载体；细菌；云母类矿物；生物风化作用
中图分类号：Q939.99
文献标识码：A
文章编号：1006-7493（2012)0117-08
IsolationofaSiderophore-ProductingBacterialStrain
andMica-bacterialInteractions
HELinyan,ZHANGYin,SHENG Xiafang,HUANGZhi
College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of
Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China
Abstract: Studies on the interactions between siderophore-producting bacteria and mica minerals will help us understand the mechanism of bio-weathering, the formation of soil, global cycle of several elements, and local environmental contamination. A siderophore(pyoverdins)-producting bacterial strain Z6 was isolated from rhizosphere soil of advantage wild plants sheep sorrel(Rumex acetosa L.) growing in Longshan potassium mine tailings in Nanjing, which was identified as Pseudomonas sp. by checking the individual morphology, colony characteristics, and 16S rDNA sequencing. Using the test cultures containing biotite or muscovite inoculated with Pseudomonas sp. Z6, we found that a strong increase in the amount of siderophore in the fiest 15 days and bacteria could influence silicon and iron mobilization from mica minerals consistently until 75 d of culture. The amounts of iron released from biotite in the presence of Z6 increased by 211 times and the silicon increased by a factor of 27.8, much higher than that in the negative control without minerals. SEM analysis revealed the formation of erosion and bacteria-mineral aggregates on the surface of mica. Cellular growth, siderophere production and pH change by Pseudomonas sp, Z6 cultivated in biotite were directly and quickly influenced, more significantly than that in muscovite experimental setup. The siderophore(pyoverdins) producting bacterial strain Z6 might play an important role in the process of mica weathering. Production of bacterial siderophore may be related to the presence of different mica minerals.
Key words: pyoverdins; bacteria; mica minerals; bio-weathering
First author: He Linyan, Associate Professor; E-mail: helyan0794@njau.edu.cn 收镐日期：2011-08-07；修国日期：20110920
基金项目：国家自然科学基金（30400006，41071173）；中国博士后基金（20090451221）联合资助
作者简介：何琳蕉，女，1976年生，副教授，研究方向：微生物资源与地质微生物学；E-mail:helyan0794@njau.edu.cn