植物营养与肥料学报2010,16(5)：1209-1216 Plant Nutrition and Fertilizer Science
NaCl胁迫对菜用大豆种子抗坏血酸-谷胱甘肽
循环的影响
王聪2，朱月林1·，杨立飞"，陈磊!
（1国家大豆改良中心，南京农业大学园艺学院，江苏南京210095；2内蒙古民族大学农学院，内蒙古通辽028042）摘要：采用蛭石赖培，在I00mmol/LNaCI胁追下，对耐盐性不同的两个菜用大豆[Glycinemax（L.）Merr.]品种种子的过氨化氢(H,O,）含量及抗坏血酸-谷胱甘肽（AsA-GSH)循环进行了研究。结果显示，NaCI胁迫显著增加了菜用大立种子的H,0，含量，但耐盐品种"绿领特早"的增幅低于盐敏感品种"理想高产95~1"。NaCI胁迫期间，"绿领特早种子中抗坏血酸过氨化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、谷胱甘肽还原酶（GR）活性，AsA、
-1"；脱复抗坏血酸(DHA)、氧化型谷胱甘肽(GSSG)含量的增幅低于同期的"理想高产95-1"。表明“绿领特早种子在迫期间能够保持较高的AsA-CSH循环效率，可有效地抑制H,O，的积累，这可能是其耐盐性较强的重要原因之。
关键调：NaCI胁迫；菜用大豆种子；抗坏血酸；谷胱甘肽
中图分类号：S565.1.01
文献标识码：A
文章编号：1008505X(2010)05-1209-08
Effectsof NaCl stressonascorbate-glutathione cycleinvegetablesoybeanseeds
WANG Cong", ZHU Yue-lin'*, YANG Li-fei', CHEN Lei
(I National Center for Soybean Improvement/Collegeof Horticulture,Nanjing Agricultural University,Nanjing 210095,China
2 College of Agronomy,Inner Mongolia Uniersity for Nationalities,Tongliao, Inner Mongolia 028042,China)
Abstract : The peroxide(H,O, )content and ascorbate( AsA)-glutathione( GSH) cycle in seeds of two vegetable soy bean cultivars[ Glycine max (L. )Merr. Jwith different salt tolerances were studied using the vermiculite culture un-der 100 mmol/L NaCl stress. The results show that H,O, contents in seeds of two vegetable soybean cultivars are significantly increased under the NaCl stress, and the increased amount of “ Lalingtezao" resistant to the NaCl stress is lower than that of "Lixianggaochan 95 1"susceptible to the NaCI stress. During the NaCl stress period, the ac-tivities of ascorbate peroxidase(APX), dehydroascorbate reductase(DHAR)and glutathione reductase(GR), con-tents of AsA and GSH, and ratios of AsA/DHA and CSH/GSSG of “Lilingtezao"are higher than those of “Lixiang gaochan 95 -1", and/or their decrease were lower than those of "Lixianggaochan 95 1". While contents of de hydroascorbic acid( DHA) and oxidized glutathione ( GSSG) of " Lilingtezao" are lower than those of " Lixiang-gaochan 95-1",These results indicate that“Lalingtezao" could maintain efficient metabolism of AsA-GSH cycle during the stress period, and further maintain lower H, O, accumilation. This might be one of important reasons for higher salt tolerance of "Lalingtezao".
Key words: NaCl stress; vegetable soybean seed; ascorbate; glutathione
收稿日期：2009-11-02
接受日期：2010-0322
基金项目：“十一·五"国家高技术863"研究计划重大项目（2006AA10A110）；国家转基因生物新品种塔育科技重大专项
(2009ZX080042011B)；内蒙古民族大学博士启动基金项目（BS217）资助。
作者简介：王聪（1968一），男，内蒙占和林格尔县人，博士，副教授，主要从事策生理和生物技术研究。E-mail；tongliaowangong@163.com
通讯作者E-mail;ylzhu@njau.edu.en
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