植物营养与肥料学报2010,16(5)：1217-1223 Plant Nutrition and Fertilizer Science
NaCl胁迫下不同砧木对嫁接黄瓜叶片
氮素代谢的影响周俊国，鹿惠灵（河南科技学院冈艺系，河南新乡453003）
摘要：采用营养液栽培，研究了以中国南瓜"360-3×112-2"杂交种和黑籽南瓜为砧木，以"津春2号”为接穗的2 种嫁接黄瓜和"津春2号"白根黄瓜成株期在80mmol/LNaCI胁迫12d后的生长状况和光合能力，以及试验期间叶片氮素代谢及其关键酶活性的动态变化。结果表明，NaCI胁迫12d后以中国南瓜“360-3×112-2"杂交种为砧本嫁接黄瓜植株的主签生长速率、第一片功能叶面积、全株鲜重和光合参数均高于自根黄瓜和以黑籽南瓜为砧木的嫁接黄瓜；且以"360-3×112-2"杂交种为砧木嫁接黄瓜的生长状况好于以黑籽南瓜为砧木的嫁接黄瓜。NaCI 胁迫处理植株叶片中硝态氮、铵态氮含量、谷氨酰胺合成酶（Gs）活性和可溶性蛋白含量比对照先高后低；而硝酸还原醇（NR）活性先低后回升，组仍低于对照，说明NaCI胁迫下，黄瓜叶片氮素代谢能力的降低。不同处理氮素代谢能力的差异其原因在于根系吸收氮源的能力差异。NaCI胁迫对以"360-3x112-2”杂交种为贴本的黄瓜植株生
长抑制较小，氮索代谢能力下降较少，表现出较强的谢盐性，可以作为一个黄瓜耐盐砧木在生产上使用。关键调：NaCI胁迫：嫁接黄瓜；砧木；中南瓜条交种；黑籽南瓜；氮系代谢
中图分类号：S642.2；S945.78
文献标识码：A
文章编号：1008505X(2010)051217-07
Effectofdifferentrootstocksonnitrogenmetabolismofgraftedcucumber
leavesunderNaCIstress
ZHOU Jun-guo,HU Hui-ling
(Department of Horticulture, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China)
Abstract: The growth and nitrogen metabolism of leaves of grafted cucumber and non-grafted cucumber at the adult plant stage were studied in hydroponic solutions under 8O mmol/L NaCI stress, and non-NaCI stress. The Cucurbita moschata hybrid of 360 -3 × 112 2 and Cucurbita ficijfolia were used as rootstocks, and cucumber cultivar,*Jinchun 2" was used as scion. The results show that the growth of grafted cucumber with C. moschata hybrids of"3603 x1122"as rootstock is better than that of C. ficifolia under the NaCl stress.The nitrate and ammonium nitrogen contents,activity of glutamine synthetase and content of soluble protein in leaves of all treatments under the NaCl stress are higher than those of the control at the prophase, then lower than those of the control at the ana-phase, while the activity of nitrate reductase is depressed at first, and then increased, but lower than that of the control all the time. These changes suggest that the ability of nitrogen metabolism of the plants is decreased under the NaCl stress, and the difference of the ability of nitrogen metabolism between all treatments is due to the root capacities of nitrogen uptake. Tested plants grafted on C. moschata hybrid of *360 3 × 112 2" has less growth inhibition, less reduce of nitrogen metabolism ability, therefore, it is a promising rootstock for high salt tolerance. Key words: NaCl stress; grafted cucumber; rootstock; Cucurbita moschata hybrid; Cucurbita ficifolia;
nitrogen metabolism
收稿日期：200910-26
接受日期：2010-01-13
基金项目：河南省重点科技攻关计划资助项目（082102150036）：阿南科技学院2008年高层次人才科研项目(08011)。
作者简介：周俊国（1967一），男，河南内乡人，教授，博士，主要从事园艺植物种质资源与育种的研究工作。E-mail：junguo1020@163.com 万方数据