植物背养与肥料学报2010,16(3)：768-772 Plant Nutrition and Ferilizer Science
氮素不同形态配比对菜用大豆生长、种子抗氧化
酶活性及活性氧代谢的影响
陈磊，朱月林*，杨立飞，王聪（南京农业大学园艺学院，江苏南京210095)
摘要：通过蛭石盆裁试验，研究了氮索不同形态配比对案用大豆[Glycinemaxr（L.）Merr.]品种"理想95-1"生长、种子抗氧化酶活性及活性氧代谢的影响。结果表明，营养液中适宜的硝铵比(75:25)有利于菜用大互的生长发育，植株具有最大生物量：在高比例的硝态氮（100%）和钱象氢（75%)处理下，植株的干重、鲜重及产量均显差降低，以确
较低；面在硝铵比为25:75处理中，抗氧化酶活性最高，0生成速率、H,0和MDA含量也最高，表明过多的铵态氮对细胞膜造成了伤害，所受的氧化损伤程度较重。
关键词：氮素形态；菜用大豆；抗氧化酶；膜脂过氧化
中图分类号：S643.7：S143.1
文献标识码：A
文章编号：1008505X(2010)03076805
Effects of nitrogen forms and ratios onplantgrowth,seed antioxidant enzyme
activitiesandreactiveoxygenmetabolismofvegetablesoybean
CHEN Lei, ZHU Yue-lin', YANG Li-fei, WANG Cong
(College of Horticulure，Nanjing Agriculwral Uniersity，Nanjing，Jiangsu 210095，China）
Abstract: Using the vermiculite culture, the effects of ratios of NO; -N and NHt -N on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean [ Glycine max (L.) Mer. ev. Li-xiang 95 1] were studied. The results show that the appropriate ratio of NO; -N and NHt -N is about 75 : 25 which is beneficial to the growth and development of the soybean, and produces the maximum plant biomass.Under the treatment of excessive NO; (100%) or NHt (75 %), both biomass production and yields are decreased obviously, especially for the NH(75%) treatment. In the NO; : NHt treatments of 75:25 and 50: 50, the activities of antioxidant enzymes are low, and the O,producing rale, hydrogen peroxide (H,O,) and malondiadehyde (MDA) contents are also low, therefore the de-gree of oxidative stress is comparatively low. However, under the NO; : NHt treatment of 25:75, the antioxidant enzyme activities, the O,' producing rate, H,Oz and MDA contents reach to their highest values, These results indicate that ex-cessive NHt is hamful to cell membrane integrity, resulting in severe degree of oxidative damage in the seeds of veg-etable soybean.
Key words: nitrogen forms; vegetable soybean; antioxidant enzyme activity; membrane lipid peroxidation
硝态氮和铵态氮是蔬菜作物吸收的两种主要氮素形态，但是不同蔬菜作物对这两种氮素形态吸收、还原、运输、分布和同化等方面是截然不同的，从而
收稿日期：2009-0527
接受日期：2009-09-21
对蔬菜的生长和代谢产生不同的生理效应[1-2]。赵建荣等3研究发现，氮素形态显著影响菠菜营养品质和抗氧化酶活性，在完全供应铵态氮时，膜脂过氧
010100）（9
作者简介：陈磊（1982—），男，重庆长寿人，博上研究生，主要从事蔬菜载培生理研究。E-mail：leichennjau@163.oom
通讯作者E-mail：ylchu@njau.edu.en
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