植物营养与肥料学报2016,22(5):1356-1365 Journal of Plant Nutrition and Fertilizel
doi: 10.11674/zwyf.15393 http://www.plantnutrifert.org
NaC1胁迫下外源壳聚糖对菜用大豆叶绿体
抗氧化系统的影响
王聪，董永义，贾俊英，包金花，马玉露，郑毅
（内蒙古民族大学农学院，内蒙古通辽028042）
摘要：【目的】叶绿体是植物体产生活性氧(ROS)、且对盐最敏感的细胞器，本试验研究了外源壳聚糖对NaC1 胁迫下菜用大豆[Glycine max(L.)Merr.]叶绿体内抗氧化系统的影响，以期探讨壳聚糖对NaCI胁迫下光合作用的调节机制。【方法】试验于2014年4月至6月在内蒙古民族大学试验基地日光温室内进行。采用蛭石裁培，选用菜用大豆盐缴感品种*理想高产95-1"(LX)、耐盐品种*绿领特早’(LL)为试材。试验设4个处理：1)叶面喷酒清水，根部浇灌营养液（对照)；2）叶面喷酒壳聚糖溶渡，根部浇灌营养液渡；3）叶面喷酒清水，根部浇灌溶有 NaC1的营养液；4)叶面喷酒壳聚糖溶液，根部浇灌溶有NaC1的营养液。【结果】外源壳聚糖显著降低了NaC1 胁迫下两品种菜用大豆叶绿体H,O的含量，显著提高了过氧化物酶(POD)、抗坏血酸过氧化物酶（APX）、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPX)活性以及胁迫中期还原型抗坏血酸(AsA)、还原型谷胱甘肽(GSH)含量；与盐敏感品种LX相比，耐盐品种LL在胁迫615d期间维持了相对较低的H,O,含量，相对较高的DHAR活性及AsA含量，在整个胁迫期间维持了相对较高的APX、GR、GPX活性，在胁迫后期(12d、15d)维持了相对较高的GSH含量。【结论】外源壳聚糖对NaCI胁迫下菜用大豆叶绿体内POD活性及AsA-GSH循环产生了显著诱导作用，但对不同品种的诱导效果不同，耐盐品种LL的AsA-GSH循环维持了相对较强的活性氧清除能力，这可能是其维持较强光合能力，进而维持较旺盛生长的重要原因之一，
关键词：外源壳聚糖；NaCI胁迫；叶绿体；抗氧化系统
中图分类号：Q945.78
文献标识码：A
文章编号：1008505X(2016)051356-10
Effectsofexogenouschitosanonantioxidantsysteminchloroplastof
vegetable soybean under NaCl stress
WANG Cong, DONG Yong-yi, JIA Jun-ying,BAO Jin-hua, MA Yu-lu, ZHENG Yi
( College of Agriculture, Inner Mongolia University for Nationalities, Tongliao, Imner Mongolia 028042, China )
Abstract: [ Objectives ] Chloroplasts are plant organelles that produce reactive oxygen species (ROS) Chloroplasts are extremely sensitive to salt stress. In order to investigate the mechanism of chitosan on regulating photosynthesis under NaCl stress, the improvement of antioxidant system in the chloroplasts by applying exogenous chitosan on soybean [Glycine max (L.) Merr.] was studied.[ Methods ] A pot experiment was conducted inside greenhouse of the Experimental Base of Inner Mongolia University from April to June 2014 Vermiculite was used as culture substrate, salt sensitive soybean cultivar of 'Lixiang gaochan 95-1* (LX) and not tolerant one of ‘Lvling* (LL) were used as test materials. Four treatments were designed: 1) Soybean leaves were sprayed with water, and roots fed with the nutrient solution (CK); 2) Only the leaves were sprayed with chitosan solution, and the roots fed with nutrient solution; 3) Leaves were sprayed with water, and the roots were fed with nutrient solution containing NaCl; 4) The leaves were sprayed with chitosan solution, and the
收稿日期：201509-23
接受日期：2016-01-21
基金项目：国家自然科学基金项目(31260472,31260483)资助。
作者简介：王聪(1968一)，男，内蒙古和林格尔县人，博士，教授，主要从事蔬菜生理生态和生物技术研究
E-mail: tongliaowangcong@163.com
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