
植物营养与肥料学报2014,20(1):221-228 Joumal of Plant Nutrition and Fertilizer
doi: 10. 11674/zwyf. 2014. 0125
缺磷胁迫下草甘麟对抗草甘麟大豆幼苗光合作用和叶绿素荧光参数的影响
原向阳,郭平毅·,黄洁,张丽光",郭俊',解丽丽"
聂萌恩",王玉国",王宏富
(1山西农业大学农学院作物化学调控与化学除草实验室,山西太谷030801;
2南京农业大学农学院,江苏南京210095)
摘要:为探明缺磷助追下草甘膦对抗草甘膦大豆幼苗叶片光合作用和叶绿素荧光参数的影响,采用溶液培养方法,在大豆长出真叶时进行缺磷胁迫,第二复叶完全展开时进行草甘脾处理,5d后测定各生理指标。结果表明:相对于正常供磷条件的清水处理,缺磷勘迫下4.98mL/L草甘麟处理的大豆叶片净光合速率(Pn)、气孔导度(Gs)、胞间CO,浓度(Ci)、最大荧光(F_)、PSⅡI最大光化学效率(F,/F_)、PSIⅡI的有效量子产量[Y(Ⅱ)]、PSIⅡI非调节性
能量耗散的量子产量[Y(NO)]、最大电子传递速率(ETR
)和半饱和光强(1,)均量下降趋势。而气孔限制值
(Ls)、叶绿素a(Chla)、叶绿素b(Chlb)、叶绿素a/b(Chla/b)、类胡萝卜素(Car)、总叶绿索(Chl)含量和PSII 调节性能量耗散的量子产量[Y(NPQ)]均呈升高趋势。说明缺磷胁追和喷施草甘瞬显著降低了抗草甘麟大豆的光合速率。缺磷引起的气孔因素可能是导致大豆光合速率下降的主要原因,而光合速率的下降导致其PSⅡ反应中
心的开放程度降低,活性减弱,参与CO,固定的电子较少,光化学效率较低。关键调:缺磷胁追;草甘膦;抗草甘麟大豆;光合作用;叶绿索荧光参数
文章编号:1008-505X(2014)01-0221-08
中图分类号:S565.1.01;Q945.78
文献标识码:A
Effect of glyphosate on photosynthesis and chlorophyll fluorescence of leaves of glyphosate-resistant soybean[Glycine max(L.)Merr.]
seedlingsunderphosphorus deficiencystress
YUAN Xiang-yang', GUO Ping-yi'', HUANG Jie', ZHANC Li-guang", GUO Jun', XIE Li-li',
NIE Meng-en', WANG Yu-guo', WANG Hong-fu'
(I Laboratory of Crop Chemical Regulation and Chemical Weed Control, Agronomy College, Shanzi Agricultural University, Taigu Shanxi 03080, China; 2 College of Agronomy, Nanjing Agricultural Unitersity,Nanjing 210095, China)
Abstract; Effect of glyphosate on photosynthesis and chlorophyll fluorescence parameters of leaves of glyphosate resistant soybean seedlings (RR1) under phosphorus deficiency stress was studied using a solution culture experiment under phosphorus deficiency stress at the true leaf stage. The glyphosate at 4. 98 ml/L was sprayed at the two-trifoliolate leaf stage, and physiological indexes were analyzed 5 days after the glyphosate spraying. The results show that under the phosphorus deficiency stress, the photosynthetic rate (Pn), stomatal conductance(Gs), intercellular CO, concentration(Ci),maximum fluorescence(F.),PSI maximum quantum yield(F,/ F.) , PSII effective quantum yield[ Y( II ) ] , PS II energy dissipation in non-regulatory quantum yield[ Y(NO)] , half saturation light intensity (Ix) and maximum electron transport rate (ETR...) in leaves of RR1 treated by 4. 98 ml/L glyphosate are lower than those treated by water under normal phosphorus condition, while the stomatal limitation value (Ls), chlorophyll a (Chl a), chlorophyll b (Chl b), chlorophyll a/b ( Chl a/b), carotenoid
收稿日期:20130509
接收日期:2013-0805
基金项目:国家引进国际先进农业科学技术"948"计划(610022);山西农业大学引进人才科研启动项目(XB2009018)资助。作者简介:原向阳(1981一),男,山西省阳城县人,副教授,博士,主要从事作物化学调控与化学除草方面的研究。
Tel; 03546289272, E-mail: yuanxiangyang200@163, com。→ 通信作者 Tel; 03546286938, Pyguo@ sxau.edu.en
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