·84·
2016 Vol.35 No.3 Serial No.289
China Brewing
纤维素酶-微波辅助法提取金樱子总臭苷的工艺研究
王慧竹'陈帅2朱岩李佳俊钟方丽*
Research Report
（1.吉林化工学院化学与制药工程学院吉林吉林1320222.沈阳药科大学药学院辽宁沈阳110016
摘要为了研究纤维素酶-微波辅助法提取金樱子中总皂苷的工艺条件以总皂苷提取率为考察指标在单因素试验基础上采用和酶用量对金樱子总皂苷提取率的影响显著而酶解温度和微波功率对总皂苷提取率无显著影响最终确定酶联微波法提取金楼子总皂苷的最佳工艺条件为酶用量0.10g/g醇解温度44℃微波时间23min微波功率470W在此工艺条件下金樱子总皂苷的提取
率预测值为6.15%实际验证值为6.11%实际值和预测值间相对误差为-0.16%充分验证了模型的稳定性和可靠性关键词纤维素酶-微波辅助法金樱子总皂苷响应面法
中图分类号R931.6
文章编号0254-5071(2016)03-0084-05
doi:10.11882/j.issn.02545071.2016.03.019
Extractiontechnologyoftotal saponinsfromRosaelaevigataebycellulase
pretreated-microwave assisted extraction
WANG Huizhu', CHEN Shuai'-, ZHU Yan', LI Jiajun', ZHONG Fanglil*
(I.School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin J32022, China,
2.School of Pharmacy, Shenyang Phamaceutica/ University, Shenyang I 10016, China)
Abstract In order to optimize the extraction conditions of total saponins in Rosae laevigatae by cellulase pretreated-microwave assisted extraction, single factor experiment was conducted with total saponin extraction rate as index, and response surface methodology was chosen to analyze the ex-periment results. The regression model of total saponin extraction rate with each influence factors was established. Results showed that the fitting de-gree of the model was good, microwave time and enzyme addition had significantly effeet on extraction rate of total saponins through the regression model while the temperature of enzymatic hydrolysis had no significant effect on the extraction rate of total saponins. The optimum process condi-tions were as follows: enzyme addition 0.10 g/g, enzymatic hydrolysis temperature 44 °C, microwave time 23 min, microwave power 470 W. Under these conditions, the predictive value for extraction rate of total saponins of Rosae laevigatae was 6.15% while the actual verification value was 6.11%. The relative error between the actual value and predictive value was -0.16%, which adequately verified the stability and reliability of the model.
Key words cellulose pretreated-microwave assisted method; Rosae laevigatae; total saponins; response surface methodology
金樱子为蔷薇科蔷薇属植物金樱子（Rosaelaevigatae）的干燥成熟果实外观呈倒卵形紫褐色外面需被刺毛 10～11月果实成熟变红时采收于燥除去毛刺。金樱子主要生于海拔100～1600m的向阳的山野、田边、溪畔灌木丛中分布于江苏、浙江、安徽、福建、江西、河南、湖北等地。2015版药典一部收载本品“金樱子为蔷薇科植物金子的干燥成熟果实性味酸、甘、涩，平。归肾、膀胱大肠经。具有固精缩尿固崩止带涩肠止泻之功能，可用于遗精滑精遗尿尿频崩漏带下久泻久痫”。现代研究表明金樱子含有多糖、有机酸、黄酮类、三菇、皂苷等多种化合物3药理实验证实金樱子具有广泛的生理活性如抗炎、抑菌、降血脂、降血压、调节免疫)、抗肿瘤、抗氧
收稿日期2015-11-30
化用等作用
由于中药化学成分多数被包要在植物细胞壁内一般
的有机溶剂难以破坏细胞壁提取率较低而纤维素酶可使细胞壁疏松、破裂从而减小传质阻力加速有效成分的释放进而提高提取效率、提高原料的利用价值。而对于总皂苷的提取目前最常用的方法是加热回流法，虽然提取率较高但提取时间较长。微波提取是利用微波能来提高提取率的一种新技术，具有省时、穿透力强、选择性高、溶剂用量少等特点(10-12)广泛应用于中药活性成分的提取本试验采用纤维素酶-微波辅助法对金樱子总皂音的提取工艺进行了研究，以期为金樱子资源的综合开发、利用提供一定参考。
作者简介主慧竹（1981-）女，讲师项士研究方向为体内药物分析，
*通讯作者钟方丽(1970-）女教授博士研究方向为天然产物化学及应用
?1994-2018 China Academic Journal Electronic Publishing House, All rights reserved.
http://www.cnki.net