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(编校：张态明）
胰腺癌干性细胞的分选及其生物学特性的初步鉴定
主
磊，胡晨曦，夏铀铀，蒋晓东
The isolation andfunctionalverificationoftumor stem cells in pancreatic adenocarcinoma Wang Lei, Hu Chenxi,Xia Youyou,Jiang Xiaodong
Department of Radiotherapy ,Lianyungang First Peoples Hospital,Jiangsu Lianyungang 222002,China.
[Abstract]
Objective: Flow cytometry was used to isolate tumor stem cells and research the culture and identifica-
tion of tumor stem cells in pancreatic adenocarcinoma. Methods: Flow cytometry was used to isolate and sort the cells into 4 group according to the cell surface markers CD44 and CD24, CD44 * CD24 * ,CD44° CD24* ,CD44 * CD24-, CD44° CD24~. ROS level was checked by DCFH - DA probe, the cells with diferent features were injected into BALB/C nu/nu mice to determine the tumorrigenicity. Results :92. 0% of sorted PANC 1 cells expressed the cell surface marker CD44,4. 7% expressed CD24. The proportion of isolated cell : CD44 * CD24 * (0. 6 ± 0. 2)% ,CD44 * CD24 ° (89.3 ±2. 6)% , CD44 ° CD24 * (4. 1 ± 1. 3)% , CD44 ° CD24° (6. 0 ± 1. 7)%. Comparing with the other groups,the ROS level of CD44 *CD24° was lower (P<0. 01). Implantation of 1 × 10* CD44°CD24° cells in nude mice,no tumor growth was ecident after 12 weeks. In contrast, nude mice impanted with 1 × 102 CD44 * CD24 * cells oedsoesaoao(aaeaaoe pancreatic cancer cells and might be the stem cells of pancreatic adenocarcinomas. Tumor stem cells of pancreatic ad-enocarcinoma have properties of a lower level of ROS.
[Key words]pancreatic adenocarcinoma,PANC1,stem cell,ROS
Modern Oncology 2015,23(22) :3214 3217
目的：应用流式细胞仪从人胰腺癌细胞系PANC-1中分选出肿瘤干性细胞，并对其生物学特性进
【摘要】
行初步鉴定。方法：流式细胞仪分选出CD44*CD24*、CD44-CD24*、CD44*CD24'和CD44°CD24-4类细胞亚群;6MVX射线照射前后，DCFH-DA探针检查各亚群活性氧簇（reactiveoxygenspecies，ROS）水平：将各亚群细胞接种于BALB/C-nu/nu操小鼠，观察、比较成瘤率。结果：4个亚群细胞比例如下：CD44*CD24*（0.6% 亚群比较，CD44*CD24*干性细胞活性氧水平在X线照射后最低，平均荧光强度（MFI)显著低于其他3组细胞(P值均<0.01）。1×10°个CD44*CD24*细胞接种于裸鼠,6周后成瘤（1/8）；接种1×10°个CD44 CD24'细胞，12周后未成瘤。结论：分选出的各亚群中CD44*CD24*更具有胰腺癌干细胞特性，体内成瘤能
【收稿日期】【基金项目】【作者单位】【作者简介】【通讯作者】
2015 05 18
江苏省连云港市科技局项目（编号：SH1218）连云港市第一人民医院放疗科，江苏连云港
222002
王磊（1982-），男，江苏连云港人，主治医师，主要从事胰腺癌的放化疗。E-mail：doctonwangl@163.com
蒋晓东（1970-），男，江苏盐城人，主任医师，博士生导师，主要从事肿瘤抗血管生长研究。E-mail;jxdysy1970@163.com