植物营养与肥料学报2014,20(1)：129-138 Joumal of Plant Nutrition and Fertilizer
doi: 10.11674/zwyf.2014.0114
不同氮素形态及其比例对菘蓝生物学特性的影响
唐晓清，肖云华，赵雪玲，周海凤，李臻颖，林志超，杨月
（南京农业大学园艺学院，南京210095）
摘要：采用砂培法栽培菘蓝，以菘蓝叶与根的干重、主根长度与直径、叶绿素含量和光合参数为指标，研究三种氮素形态，即硝态氮(NO,-N）、铵态氮（NH;-N）和酰胺态氮[CO（NH,),-N]四种不同浓度对菘蓝生物学性质的影响，为菘蓝栽培生产中高效利用氮肥提供理论依据。结果表明，氮素处理能增加菘蓝叶与根的干重，铵态氮对菘蓝主根长度及侧根数的影响最显著，而酰胺态氮与硝态氮对主根直径的影响相近，硝态氮对菘蓝的叶干重、根干重与根冠比的影响最为显著；不同形态氰素索处理能显著提高叶绿素含量与净光合建率，硝态氮对叶绿素和净光合速率有显著影响，酰胺态氮对气孔导度与燕膀速率的影响最显著，铵态氮对叶片的胞间二氧化碳浓度有显著影响。不同形态氮素通过对菘蓝叶片的叶绿素含量和光合参数的影响实现对其生长状况的影响，其中硝态氮对菘蓝生长的影响最为显著。
关键词：菘蓝；氮素形态；生物学特性
中图分类号：S567.2.062
文献标识码：A
文章编号：1008505X(2014)01012910
Effectofdifferentnitrogenformsandtheirproportion onbiologicalcharacteristicsofIsatisindigoticaFort
TANG Xiao-qing, XIAO Yun-hua, ZHAO Xue-ling, ZHOU Hai-feng, LI Zhen-ying, LIN Zhi-chao, YANG Yue
(College of Horticulture, Nanjing Agricultural Unitersity,Nanjing 210095, China)
Abstract: To provide a theoretical basis of efficient utilization of nitrogen fertilizer for cultivation and production of Isatis indigotica, effects of different nitrogen forms and their proportion on biological characteristics of I. indigoticd were studied by using sand culture. There were four different concentrations of three nitrogen forms, NH,'-N, NO,~-N and CO( NH, ),-N. The dry weights of leaves and roots, lengths and diameter of taproots, chlorophyll content and photosynthetic parameters were used as indicators. The results show that the nitrogen treatments could increase the dry weights of leaves and roots, the effect of NH, * -N on taproot length and number of lateral root is the most significant, the effect of amide nitrogen and nitrate nitrogen on taproot diameters is similar, and the effect of NO, --N on dry weights of leaves and roots and root/shoot ratio is the most significant. The nitrogen treatments could significantly improve the chlorophyll content and net photosynthetic rate which affect chlorophyll and net photosynthetic rate of nitrate nitrogen, stomatal conductance and transpiration rate of amide nitrogen, and the concentration of carbon dioxide in the intercellular of ammonium nitrogen, respectively. Different nitrogen forms affect the growth through the effects of chlorophyll content and photosynthetic parameters, and in which nitrate nitrogen is the most significant nitrogen form.
Key words: Isatis indigotica Fort. ; nitrogen forms; biological characteristics
氮素作为一种植物生长必需的营养物质，既是植物体最重要的结构物质，又是其生理代谢中最活
收稿日期：20130528
接收日期：2013-1008
跃、无处不在的重要物质一酶的主要成分(0)，所以氮素对植物生理代谢和生长有重要作用。在所有必
基金项目：国家自然科学基金（31171486）：国家大学生创新创业训练计划（201310307026）；南京农业大学校级大学生创业训练项
目（12ey04）资助。
作者简介：唐晓清（1970一），女，川威远人，博士，副教投，主要从事药用植物栽培与中药质量研究。E-mail：xqtang@njau，edu.en 万方数据