MODERN ONCOLOCY,Mar.2012,VOL.20,NO.03
现代肿瘤医学2012年3月第20卷第3期
Shanmiao Gou, Tao Liu, Chunyou Wang, et al. Establishment of
【91]
clonal colony forming assay for propagation of pancreatic cancer
· 489·
cells with stem cell properties[J]. Pancreas,2007,34(4) :429
435.
(编校：何妹）
新型左旋棉酚纳米微球的制备及在体外抑制人前列腺癌PC-3细胞生长的研究
张献清，唐永华”，穆士杰，陈晨"，刘志新"，胡兴斌"，安群星"，黄晓峰
Preparationofanovelnanoparticlesof（-)-gossypolandgrowthinhibitiononhuman prostate cancer PC -3 cells in vitro
e HUANGXiaofeng
'Department of Blood Transfusion,Xjing Hospital, Fourth Military Medical Uniwersity,Shaanxi Xian 710032, China ;'School of Life Sci ence and Techenology, Xidian Unitersity , Shaanxi Xi'an 710071, China;"Central Laboratary, Fourth Military Medical University, Shaanxi Xian 710032,China.
[ Abstract] Objective: To investigate the antitumor effects and the possible mechanism of ( ) gossypol loaded nanoparticles with mPEC maleimide in vitro. Methods: The ( ) gossypoi loaded nanoparticles were prepared by emulsion - evaporation method. The toxicity of blank nanoparticle was measured on human prostate cancer PC 3 cells and human prostate RWPE 1 cells. The antitumor effects on PC 3 cells of nanoparticles were evaluated by MTT assay, AO staining and transmission electron microscopy in vitro,a nd then compared with free ( ) gosssy pol. , The Bcl 2 and Bak mRNA level were measured by semiquantitative RT PCR. Results: The growth inhibition esauaa aaaae eo( a of free ( ) gossypol. The nanoparticles can induce morphological change of apoptosis on PC 3 cells and down regulate Bel 2 mRNA and up regulate Bak mRNA level. Blank nanoparticals have no obvious toxicity on PC 3 cells and RWPE 1 cells in high dose. Conclusion; The ( ) ~ gossypol loaded nanoparticles had favorable antitu-mor activity and no toxicity. It can induce apoptosis of prostate cancer cells and it would be a potent antitumor nano-drug-
[Key words]() gossypol;nanoparticle;apoptosis;prostate cancer
Modem Oncology 2012,20(03) :0489 0492
）目的：研究负载左旋棉酚的单甲氧基聚乙二醇-马来酰亚胺（mPEG-mal)纳米微球的体外抗肿瘤
【摘要】
活性，并对其机理进行探讨。方法：采用乳化-挥发法制备负载左旋棉酚的纳米微球，在人前列腺癌PC-3 PCR检测Bcl-2及BakmRNA的表达等方法评价其在体外对前列腺癌细胞株PC-3的抗肿瘤活性，并与左旋棉酚裸药进行比较。结果：左旋棉酚载药微球对体外培养的前列腺癌细胞生长的抑制与裸药相似，并呈剂量-时间依赖性，纳米微球可以诱导PC-3细跑发生典型的凋亡形态学改变，使Bcl-2mRNA表达水平降低，BakmRNA表达水平升高，空白微球在高浓度时，对于肿瘤细胞系及人前列腺RWPE-1细胞株均无明显毒性。结论：左旋棉酚纳米微球具有良好的抗肿瘤活性，载体无毒，具有良好的生物相容性，能诱导前列腺癌细胞的体外凋亡，是一种具有潜力的抗肿瘤纳米药物。
【收稿日期】【修回日期】【作者单位】
【作者篇介】【通讯作者】
2011 07 15 2011 08 10
1第四军医大学西京医院输血科，陕西西安710032
3西安电子科技大学生命科学学院，陕西
西安
710071
第四军医大学中心实验室，陕西西安
710032
张献清（1965-），男，湖南人，博士，副教授，主要从事肿瘤分子药理学研究。E-mail；zhangxq@fmmu.edu.cn 黄晓峰（1963-），男，安徽人，博士，教授，主要从事超微病理研究。E-mail：huangxf@fmmu.edu.en