第36卷，第5期 2016年5月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol. 36,No. 5+pp1585-1591
May,2016
Interaction Study of Ferrocene Derivatives and
HemebyUV-VisSpectroscopy
HAN Guo-cheng,FENG Xiao-zhen,LIANG Jin-tao,XIAO Wen-xiang,CHEN Zhen-cheng
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004 , China
AbstractThe interaction between ferrocene derivatives+ such as Fc(COOH), (Am =286 nm), Fc(OBt), (A..) =305 nm),Fc(Cys)(Amx=289 nm) and heme(amx=386 nm) were studied by UV-Vis spectroscopy, respec tively. The results show that, when the concentration of heme is fixed, the absorbance of heme increases with the increase of Fc(COOH): and Fc(Cys) concentration, the absorbance of heme almost keep the same when Fc(OBt) concentration increases: when the concentration of ferrocene derivatives are fixed, the absorbance of Fc(COOH); and Fc(Cys) also increases with the increase of heme concentration, the absorbance of Fc(OBt); almost keep the same when heme concentration increase. It is demonstrated that the hydrogen bonding interac tions happen between Fc(COOH)2 , Fc(Cys) and heme, none of Fc(OBt)2 + the formation of hydrogen bonding lead to the growth of molecular chain, the bigger molecule can absorb more energy and increase the absor-bance. Meanwhile, the stability of molecule is affected by the formation of hydrogen bonding, when the reac tion time increases from 0. 5 h to 18 h and 48 h, the absorbance at Amx =384 nm change from 2. 64 to 2. 53 and 2. 51 with fixed concentration of Fc(COOH) , the absorbance at Anx =384 nm change from 1. 76 to 1. 72 and 1, 68 with fixed concentration of heme, the absorbance at Amux =397 nm change from 2. 74 to 2. 63 and 2. 55 with fixed concentration of Fc(Cys) , and the absorbance at Am =397 nm change from 1. 82 to 1. 58 and 1. 49 with fixed concentration of heme, respectively.
KeywordsUV-Vis spectroscopy; Ferrocene derivatives; Heme;Hydrogen bond
中图分类号：0657.3
Introduction
文献标识码：A
D0I: 10. 3964/j. issn.10000593(2016)05-1585-07
reported. These methods inelude capillary electrophoresis[s], electrochemistryls},spectrophotometry?],spectrofluorime tryla-9], chemiluminescenceo, and high performance liquid
Hemoglobin can transport oxygen in higher organisms, this kind of protein is composed of four chains, two and two chains, each chain has a ring of heme, which is the active center of hemoglobin. It is also related with many clinical diseases, such as diabetes, leukemia, anemia and heart disea-ses.2-). Therefore, it has great significance to quantitative de-tect hemoglobin. Several analytical methods for detecting he moglobin in pharmaceutical or biological systems have been
Received: 2015-01-15 ; accepted: 2015-04-21
chromatography[11 and so on.
It is well known that ferrocene (Fc) is an organometallic compound consisting of two cyclopentadienyl rings bound on opposite sides of a central metal Fe atom. The rapid growth of organometallic chemistry is attributed to the discovery of ferrocene and its many derivatives. Most importantly, ferro cene and its derivatives have lots of applications, for exam ples, they can be used in asymmetric catalysis and enantiose
Foundation item: The National Natural Science Foundation of China (61301038), Natural Science Foundation of Guangxi Province
( 2015GXNSFBA139041 ),National Undergraduate Training Programs for Innovation and Entrepreneurship(201510595044)
Biography : HAN Guo-cheng, (1981—) , assoicate professor, School of Life and Environmental Sciences, Guilin University of Electronic Tech-
nology
e-mail: hangc81@guet. edu. cn
Corresponding author
e-mail: chenzhcheng@163.com