植物营养与肥料学报2011,17(2)：341-348 Plant Nutrition and Fertilizer Science
半定量RT-PCR研究氮素形态对樱桃番茄果实中
有机酸代谢相关酶基因表达的影响李庆余，徐新娟，朱毅勇，董彩霞！*，沈其荣
（1南京农业大学资源与环境科学学院，江苏南京210095；2河南科技学院生命科学学院，河南新乡453003）摘要：研究了全硝（100%N0,）、铵硝配施（75%NO,：25%NH，）及全铵（100%NH：）营养对楼桃番茹果实有机酸含量的影响,测定了与有机酸合成相关的磷酸烯醇式丙酮酸羧化酶（PEPC)、柠橡酸合成酶（CS）、苹果酸脱氢酶(MDH)活性变化，并利用RT-PCR检测了相应的基因在转录水平上的表达差异。结果表明，1）全硝和铵硝配施处理下果实柠橡酸含量在整个生育期均显著高于全铵处理，而铵硝配施处理下果实中柠橡酸和草果酸含量在成熟期均显著低于全硝处理。2)不同形氮素及配施处理下，果实中柠橡酸含量与CS活性的变化趋势均望单蜂型，但到达峰值的时间不同。全铵处理下果实草果酸含量与PEPC活性呈显著正相关，全硝、铵硝配施处理下果实草果酸和柠橡酸含都与MDH活性呈显著正相关。3）在膨大期和成热期，全硝处理和铵硝配施处理下PEPC基因(PPCI、PPC2)表达显著高于全铵处理；线粒体苹果酸脱氢酶（mMDH)的表达对果实苹果酸脱氨酶活性和苹果酸积累起主要作用，表明适当比例的铵硝配施可能通过影响果实中PEPC和MDH的活性和基因表达从面显著降低成熟果实有机酸的含量。
关键调：氮素形态；樱桃番茄；有机酸代谢基因表达；RT-PCR
中图分类号：S143.1；S641.2
文献标识码：A
文章编号：1008-505X(2011)02-0341-08
Organicacidmetabolismandrelatedgenesexpressionincherrytomatofruit
underdifferentnitrogenfertilizationusingsemi-quantitativeRT-PCR
LI Qing-yu', XU Xin-juan’, ZHU Yi-yong" , DONC Cai-xial*, SHEN Qi-rong
(1 College of Resources and Enrironmental Science, Nanjing Agriculural University, Nanjing 210095, China
2 College of Life Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China)
Abstract: Cherry tomatoes were grown in a matrix-nutrient solution co-culture systems with 100% nitrate (NO,), 100% ammonium (NH+ ) and 75% NO, :25% NH, as the N source. The effects of the nitrogen (N) forms on the contents of organic acids, the activities of phosphoenolpyruvate carboxylase (PEPC), citrate synthase (CS) and malate dehydrogenase ( MDH), and their transcriptional expression patterns were investigated during the develop ment of the fruit. The results are as follows: 1) During the development of the fruit, citrate contents under the treatments of 100% NO, and 75% NO;:25% NH, are always significantly higher than that under the treatment of 100% NH, , while the contents of citrate and malate in mature fruits under the 75% NO;:25% NH, treatment are lower than those of the 100% NO, treatment. 2) During the development of the fruit, the citrate content is related to the activity of CS under all treatments and shows the tendency with a single peak, although the peak values are different at the different stages. The content of malate is positively correlated with PEPC activity under the 100% NH, , in contrast, the contents of malate and citrate are positively correlated with MDH activity under the 100% Bs(PPCI and PPC2) supplied by 100% NO; and combined 75% NO; with 25% NH, are both significantly highe
收稿日期：2010-04-20
接受日期：2010-10-19
基金项目：国家自然科学基金项目(30600382)；土壤-植物营养国家重点实验室基金项目(055131)资助。
作者简介：李庆余（1983一），男，山东潍坊人，硕士研究生，主要从事植物营养生理方面的研究。E-mail：2007103110@nja.edu,cn
→通R作者 Tel; 02584396393, E-mail; cxdong@njau. edu.cn
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