·52·
2014 Vol.33 No.3 Serial No.265
China Brewing
内生黑曲霉产脂肪酶条件研究及其粗酶酶学特性
何茹刘娅*谢晓露”王陈强
Research Report
（1.石河子大学食品学院新疆石河子8320002.新疆冠农果茸集团股份有限公司技术中心新疆库尔勒841000）
摘要针对新疆酸酒葡萄中获得的1株产脂肪酶的内生黑曲霉菌株C2J6研究了该菌株的产醇条件及酶学特性。结果表明该菌适宜的产酶条件为1%的乳糖为碳源，1%的蛋白陈为氨源培养基初始pH值为8.0培养温度35℃培养时间约72h此时所产脂肪醇的活力可达18.75U/mL。该菌所产脂肪酶粗酶液的最适反应温度为40℃最适反应pH值为7.0为中温中性酶在50C保温1h酶活力保留 54.55%具有良好的热稳定性在pH值3.0-7.0范围内较稳定有一定的耐酸性。金属离子Mn对酶活力有促进作用Zn*、Fe*、Cu对醇活力有抑制作用K*、Ca*Na*、Mg~对酶活力影响不大。该醇在以蔓花油和谷物调和油为底物时酶活分别为228%和180%明显高于澈榄油和油烟机暖油
关键词内生黑曲霉脂肪醇产酶条件酶学特性
中图分类号TQ925
文献标识码A
doi :10.3969/j.issn.02545071.2014.03.014
文章编号0254-5071(2014)03-0052-05
ConditionsoflipaseproductionbyendophyticAspergillusnigerand enzymatic
properties of the crude enzyme
HE Ru', LIU Ya *, XIE Xiaoxia', WANG Chenqiang
(I.College of Food Science, Shihezi University, Shihezi 832000, China;
2. Technology Center, Xinjiang Guannong Fruit & Antler Group Co., Ltd., Korla 8410oo, Chinaj
Abstract: A lipase-producing strain Aspergillus niger C2J6 was screened from Xinjiang wine grape. The conditions for enzyme production and enzy matic properties of C2J6 were studied. The results showed that the suitable conditions for enzyme production were as follows: lactose 1% as carbon source, peptone 1% as nitrogen source, initial pH 8.0, culture temperature 35C, incubation time 72 h. Under the optimal condition, the lipase activity reached 18.75 U/ml. The enzyme was medium temperature amylase, and the optimal reaction temperature, pH of crude enzyme produced by the strain was 40°C and 7.0, respectively. It had good thermal stability, 54.55% of the enzyme activity could be reserved after being kept at 50°C for 1 h Its activity was stable between pH 3.0-7.0, and it had some acid-resisting features. Mn could boost up enzyme activity, whereas Zn*, Fe*t, Cu* would restrain its activity, and K, Ca*t, Na*, Mg* have little effect on its activity. When the enzyme was in sunflower oil and grain as substrate, the
enzyme activity was 228% and 180%, respectively, which was signifieantly higher than the olive oil and smoke lampblack machine oil. Key words; endogenous Aspergillus niger; lipase; enzyme producing condition; lipase properties
脂肪酶(EC3.1.1.3)又称三酰基甘油酰基水解酶可将甘油三酯分解成脂肪酸和甘油或进行其逆反应在油-水界面及非水介质中催化酯的水解和合成酯交换催化生物表面活性剂、高聚合物、多肽和手性药物的有机合成反应等川。目前脂肪酶已广泛应用于食品、香料、医药、化妆品、洗涤剂、皮革、药物合成、有机合成等领域2-]。微生物脂肪酶因其具有较动植物脂肪酶更好的稳定性、选择性、底物特异性、更广的作用pH值和温度范围且微生物来源的脂肪酶一般都是分泌性的胞外酶便于工业化大生产和获得高纯度样品因此成为工业脂肪酶的最重要来源。由于
收稿日期2014-01-19
基金项目新疆石河子大学高层次人才启动项目(RCZX20109) 作者筒介何茹（1987-）女硕士研究生研究方向为果蔬加工
植物内生环境这个微生态体系的特殊性,其中的微生物脂肪酶也可能表现出与众不同的特性。已有研究表明植物内生菌脂肪酶不仅具有普通脂肪酶的一些性质,而且有一些特殊的性质（如耐热、耐有机溶剂、耐碱等）是挖掘和分离新型工业用脂肪酶的潜在理想源泉5)。新疆地处祖国西部边睡多样化的气候和环境条件势必造成新疆葡萄内生菌资源的特异性和多样性。本研究针对从新疆酿酒葡萄内生菌中筛选获得的一株产脂肪酶的高活力菌株—黑曲霉(Aspergillusniger)C2J6，了解其产脂肪酶条件并对其粗酶学特性进行研究(-14)从而为该菌的开发利
*通讯作者刘娅（1974-）女副教授博士研究方向为农产品贮藏与加工
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