.8.
2010 No.4 Serial No.217
ChinaBrewing
五碳糖发酵生产乙醇的菌种研究进展
贺应龙1-2，熊兴耀1.2*，苏小军2
Forum and Summary
（1.南农业大学园艺园林学院，湖南长沙410128;2.作物种质创新与资源利用国家重点实验室培养基地，潮南长沙410128）摘要：当前以木质纤维素为原料制备燃料乙醇已成为全球研究热点，发酵五碳糖产乙醇的菌种选育是其关键技术之一，文中综述了微生物发酵五碳糖产乙醇的机理，五碳发酵自然微生物的种类及菌种遗传改良的研究进展，并对今后菌种的研究方向进行了简要论述，
调：五碳糖：乙醇；发醇；改良
关
键
中图分类号：TQ223.1
文献标识码：A
文章编号：0254-5071(2010)04-000804
Researchprogressinmicroorganismforethanol fermentationbypentose
HE Yinglong2, XIONG Xingyaol2*, SU Xiaojun*
(I.College of Horticulture and Landscape, Hu'nan Agricultural University, Changsha 410128, China; 2.Pre-StateKeyLaboratory forGermplasm Innovation and Utilization ofCrop,Changsha410128,China)
Abstract: Bioconversion of lignocelluloses to ethanol has become a global research focus in recent years. The breed of microbes for fermenting pen-tose to ethanol is one of the key technologies. The mechanism of bioconversion of pentose to ethanol is introduced in this paper. The advances on the
natural strains of pentose fermentation and genetic improvement were reviewed. In addition, the research prospect was discussed. Key words: pentose; ethanol; fermentation; improvement
由于石油等化石能源的大量开采，消耗过快，石油资
源储备已经到了临枯竭的地步。因此，寻找一种化石能
收稿日期：2009-12-23
基金项目：国家重点基础研究发展计划(973计划）(2009CB226108)
作者简介：贺应龙(1985-)，男，湖南宁多人，在读硕士研究生，主要从事生物质能源研究工作；熊兴耀*，教授，通讯作者。
biosynthesis[J]. Sci, 1999, 284(5418):1368-1372.
[14] HENDRICKSON L, DAVIS C R, ROACH C, et al. Lovastatin
biosynthesis in Aspergillus terreus: Characterization of blocked mutants enzyme activities and a multifunctional polyketide synthase gene[J]. Chem Biol, 1999, 6(7): 429-4391.
[15] AUCLAIR K, KENNEDY J, HUTCHINSON C R, et al. Conversion of
cyclic Nonaketides to lovastatin and compactin by a lovv deficient mutant of Aspergillus terreus [1]. Bioorg Med Chem Lett, 2001, 11(12):1527-1531,
[16] MANZONI M, ROLLINI M. Biosythesis and biotechnological
production of statins by filamentous fiungi and application of these cholesterol-lowing drugs[J]. Appl Microbiol Biotechnol, 2002, 58(5): 555-564.
[17] HUTCHINSON C R, KENNEDY J, PARK C, et al. Aspects of the
biosynthesis of non-aromatic fiungal polyketides by iterative polyketide synthases[]. Anton Leeuw, 2000, 78(3-4): 287-295.
[18] CHEN Y P, LIAW L L, WANG C L, et al. Monascus pilosus monacolin
K biosynthetic gene cluster, complete sequence. Direct Submission(23AUG2005)[DB]/序列号: DQ176595, Genebank[13092005]-
[19] CHEN Y P, TSENG C P, LIAW L L, et al. Cloning and characterization of Monacolin K biosynthetic gene cluster from Monascus pilosus[1], J Agric Food Chem, 2008, 56(14): 5639- 5646.
[20] SAKAI K, KINOSHITA H, NIHIRA T. Identification of mokB involved
in monacolin K biosynthesis in Monascus pilosus[]. Biotechnol Lett, 2009, 31(12): 1911-1916.
兰,Monascus ruber5031洛伐他汀合成酶基因的PCR分析[1]
[21]高
广东药学院学报，2006,22(1)：82-84.
[22]黄欣，朱碧云，吕带第，等.落伐他汀合成酶调控基因lovE和mkH 的克隆及其序列分析比较[1].广东药学院学报，2009,25(1):83-86.
[23] SCHUMANN J, HERTWECK C. Advances in cloning, fiunctional
analysis and heterologous expression of fungal polyketide synthase genes[J]. J Biotechnol, 2006, 124(4);: 690-703.
[24] RANGANATHAN A. Process for the preparation of simvastatin [P].
WO/2003/010324
[25] MEYER V, Genetic engineering of filamentous fungi-progress, obstacles
and future trends[J]. Biotechnol Adv, 2008, 26(2): 177-185.
[26]夏永军，牛丹丹，王正祥，等，红曲菌9903A转化体系影响因素的研
究[1].微生物学,2007,34(2)279-282.
[27]周礼红，王正祥，诸葛键.红曲毒不同转化方法的比较[].遗传，2006,
28 (4) ;479-485.
[28]邵总春，王汝毅，丁月娣，等.农杆菌介导的红曲荫T-DNA插入突变
库的构建及色素突变子的性质分析[],菌物学报,2006,25(2);247-255[29] CAMPOY S, PEREZ F, GUTOERREZ S, et al. Stable transformants of the azaphilone pigment-producing Monascus purpureus obtained by protoplast transformation and Agrobacterium-mediated DNA transfer[]. Curr Genet, 2003, 43 (6): 447-452.
[30] Flor Perez. Stable transformants of the azaphilone pigmentproducing
pue bogeosuen sedrod q poueq sndind snaseuo Agrobacterium-mediated DNA transfer[J]. Curr Genet, 2003, 43 : 447-452
[31] KIM J G, DO CHOI Y, CHANG Y ], et al. Genetic transfommatioe of
Monascus purpureus DSM 1379[J]. Biotechnol Lett, 2003, 25(18): 1509-1514.
[32] JIA X Q, XU Z N, ZHOU L P, et al. Elimination of the mycotoxin
citrinin production in the industrial important strain
Monascus
purpureus SM001[1]. Metab Eng, 2010, 12(1): 1-7.
蓝，李浩明.红曲毒聚酬类次生代谢产物的生物合成[].食品工
[33]高
业科技，2008,29(3)；292-295