第34卷，第6期 2014年6月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol, 34,No, 6,pp1512-1517
June,2014
AnalysisofPoly-B-Hydroxyalkonates（PHA)DuringtheEnhanced Biological Phosphorus Removal Process UsingFTIR Spectroscopy
LI Wei-hual-2, MAO Qin-yan',LIU Yi-xin', SHENG Guo-ping*, YU Han-qing",
HUANG Xian-huai',LIU Shao-geng',LING Qi',YANGuo-bing
1. School of Environment and Energy Engineering, Key Laboratory of Anhui Province of Water Pollution Control and Wastewater Reuse, Anhui Jianzhu University, Hefei 230601, China
2. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
Abstract Enhanced biological phosphorus removal (EBPR) is the main phosphorus removal technique for wastewater treatment. During the anaerobic-aerobic alternative process, the activated sludge experienced the anaerobic storage of polyhydroxy-β-alkonates (PHA) and aerobic degradation, corresponding the infrared peak intensity of sludge at 1 740 cm1 increased in the aerobic phase and declined in the anaerobic phase. Compared with PHA standard, this peak was indentified to attribute the carbonyl of PHA. The overlapping peaks of PHA, protein I and II bands were separated using Gaussian peak fitting method. The infrared peak area ra tios of PHA versus protein I had a good relationship with the PHA contents measured by gas chromatography, and the correlation coefficient was 0. 873. Thus, the ratio of the peak area of PHA versus protein I can be considered as the indicator of the PHA content in the sludge. The infrared spectra of 1 480~1 780 cm1 was selected, normalized and transferred to the absorption data. Combined with the chromatography analysis of PHA content in the sludge sample, a model between the Fourier-transform infrared spectroscopy (FTIR) spec-tra of the sludge and PHA content was established, which could be used for the prediction of the PHA content in the unknown sample. The PHA content in the sludge sample could be acquired by the infrared spectra of the sludge sample and the established model, and the values fitted well with the results obtained from chromato-graph. The results would provide a novel analysis method for the rapid characterization and quantitative deter mination of the intracellular PHA content in the activated sludge.
Keywords Infrared spectra; Poly-β-hydroxyalkonate; Gaussian peak fitting; Partial least squares
中图分类号：TQ351.2
Introduction
文献标识码：A
D0I: 10. 3964/j. issn. 1000-0593(2014)06151206
process, and it is economically efficient and environmental friendly. It became the main technical tools for the removal of phosphorus in the municipal wastewater(1], The phosphate
The phosphorus content was correlated to the eutrophi-cation and phosphorus removal from sewage and industrial wastewater has been considered as a key strategy in preven-ting eutrophication. It does not need to add the chemical rea-gents in the enhanced biological phosphorus removal (EBPR)
Received: 2013-09-02; accepted: 2013-12-12
removal was achieved by the disposal of sludge at the end of the aerobic phase through the alternate operation of anaerobic and aerobic phase.
Poly-g-hydroxyalkonates (PHAs) play an important role in the biological phosphorus removal process as an intracellu-
Acknowledgements: The NSFC (Grant No. 21007001, 51278002), and the Key Special Program on the S&.T for the Pollution Control and
Treatment of Water Bodies of China (2012ZXo07103001)
Biography: LI Wei-hua, (1977—), Ph. D, , Associate professor of Anhui Jianzhu University
+ Corresponding author
e-mail: gpsheng@ ustc. edu. cn
e-mail: weihuali@mail. ustc, edu. cn