植物营养与肥料学报2013,19(1)：134-14) Plant Nutrition and Fertilizer Science
doi:10.11674/zwyf.2013.0116
不同pH条件下腐植酸对土壤中砷形态
转化的影响
刘利军12，洪坚平1*，闫双堆'，党晋华2
（1山西农业大学资源环境学院，山西太谷030801；2山西省环境科学研究院，山西太原030027）
摘要：为了探索在不同pH条件下腐植酸对土壤中砷形态转化及生物毒性的影响，应用油菜盆裁试验，检测砷加人土壤后15d、45d、90d土壤中AE-As（水溶态、可交换态和碳酸盐结合态)Fe，Mn-As（铁/锰氧化物结合态），O，S-As(有机物及硫化物结合态)，Res-As(残渣态)的含量及油菜生物量。结果表明，外源水溶态砷加人土壤后均迅速向相对稳定的形态转化，15d时A3H1（pH9.5，腐殖酸用量为0g/kg）和A3H4(pH9.5，腐殖酸用量20g/kg)处理的AE-As含量分别为13.40mg/kg、9.23mg/kg，转化率分别为78.7%、82.51%；在90d时，A3H4处理的AE-As、 Fe，Mn-As含量分别为6.28mg/kg、1.23mg/kg.仅为处理A3H1处理的53.9%和10.7%，0,S-As、Res-As含量分别为20.24mg/kg、41.21mg/kg，是处理A3H1的165%和127%。说明离植酸更有利于土壤中AE-As向其他相对稳定的形态转化，主要体现在O,S-As的增加，腐植酸对碱性土壤中碑向较稳定形态转化的促进作用更加显著，面且与腐植酸的施用量正相关。外源砷在碱性条件下对植物的毒害更大，腐植酸用量在10g/kg土时就可以有效降低砷的毒害。
关键词：腐植酸；pH；砷形态；生物毒性
中图分类号：S143.92；S153.6*1
文献标识码：A
文章编号：1008505X(2013)01-0134-08
EffectsofhumicacidontransformationofsoilAsindifferentpHconditions
LIU Li-jun'-2, HONG Jian-ping'', YAN Shuang-dui', DANG Jin-hua?
(I College of Resourees and Environmental Sciences, Shanxi Agricultural Unirersity, Taigu, Shanxi 03080I,China;
2 Shanxi Research Academy of Enwironmental Science, Taiyuan 030027, China)
Abstract: Rape cultivated in pot was used to investigate the effects of humic acid on the form transformation and biotoxicity of As in soils. The contents of AE-As, Fe, Mn-As, O, S-As and Res-As in soils and the rape biomass were measured on the 15**, 45 and 90 day. The result shows that the exogenous hydrosoluble As is rapidly transformed to a relatively stable form in soils. After 15 days, the AE-As contents of A3H1 (pH 9. 5,humic acid rate is 0 g/kg) and A3H4(pH 9. 5,humic acid rate is 20 g/kg ) are 13. 40 and 9. 23 mg/kg respectively, the conversion rates reach up to 78.7% and 82.51%. On the 90* day, the contents of AE-As and Fe, Mn-As of A3H4 are 6. 28 and 1. 23 mg/kg, respectively. Which account for only 53. 9% and 10. 7% of A3H1. The contents of O, S-As and Res-As are 20. 24 and 41. 21 mg/kg, which are 165% and 127% of A3H1. These results suggest that humic acid facilitates the AE-As transform into other stable forms, and the most obvious change is the increase of O, S-As. Humic acid demonstrates a more significant promotive effect when the soil is alkaline, which is positively correlated with the humic acid consumption. The exogenous As has a stronger toxicity to plants in alkaline
conditions. The dosage of humic acid at 10 g/kg soil is effective to decrease the As toxicity. Key words: humic acid; pH; arsenic form; biotoxicity
收稿日期：2012-0525
接受日期：201208-14
基金项目：山西省青年科技研究基金（2009021007-2）；山西省高校科技研究开发项目（20091010）：山西省科技攻关项目（201101100200）
资助。
作者简介：刘利军（1974一），男，山西原平人，博士，工程师，主要从事污染土壤修复技术研究。E-mail；jysd@126.com
●通信作者Tel;03546288322，E-mail:hjpsx@ yahoo.com.cn
万方数据