植物营养与肥料学报2010,16(5)：1190-1195 Plant Nutrition and Fertilizer Science
不同施肥量条件下AM真菌对烟苗生长及
营养状况的影响
李建伟12，江龙3，袁玲2，黄建国2·
（1贵州省烟草科学研究所，贵阳550018；2西南大学资源环境学院重庆北碚400716；
3贵州大学生命科学学院，贵阳550025）
摘要：试验设置不同施肥量，于播种期分别接种根内球囊每菌（GlomusintraradicesSmith和Schenck，BEG193）和幼套球囊霉菌（GlomuseetunicatumBecker和Gerdemann，BEG168），探索了利用烤烟漂浮育苗技术生产菌根化烟苗和培育壮苗的可能性。结果表明：随着养分供应量的减少，AM真菌的侵染率提高，BEG193的侵染率高于BEG168。减施肥料总体上抑制烟苗生长，使烟苗生物量降低；但接种AM真菌显著促进烟苗生长，烟苗平均生物量比不接种的处理增加了74.38%（BEC168）和48.32%（BEG193）。接种BEC168使烟菌氮、磷、钾含量显著增加；接种 BEG193主要是提高了烟苗含磷量。在施肥量减少75%辅以少量追肥的情况下，接种BEG168之后，烟苗生长状况和磷、钾含量超过或与常规施肥的非菌根苗相似。因此，在集约化烤烟漂浮育苗过程中，可采用接种AM真菌 BEC168的方式培育壮苗，同时降低施肥景，减轻度弃营养液产生的环境污染。此外，接种AM真菌显等提高基质
中有机磷的吸收利用，这可能是菌根烟首含磷量提高的重要原因之一关键词：AM真菌；烤烟；生长；营养
中图分类号：S572.062
文献标识码：A
文章编号：1008505X(2010)05-119006
Influences of arbuscular mycorrhizal fungi on growth and nutrition of
tobaccoseedlingsunderdifferentfertilizerlevels
LI Jian-wei'-2, JIANG Long'3, YUAN Ling', HUANG Jian-guo**(I Guizhou Tobacco Research /nstitute, Guiyang 550018, China;
2 College of Resources and Enrironment, Southwest University, Beibei, Chongqing 400716, China
3 College of Life Science, Guizhou University,Guiyang 550025, China)
Abstract: Tobacco seedlings were inoculated with and without arbuscular mycorhizal fungi(AMF)at the sowing stage and grown in the culture boxes floating on the nutrient solutions to investigate the possibility of producing my-corrhizal and healthy seedlings. AMF used in the present experiment were Glomus intraradices Smith and Schenck(BEG193)and Glomuse etunicatum Becker and Gerdemann(BEG168). The results show that the colonization rate of BEG193 is significantly higher than that of BEG168, and both of them are increased as the concentrations of nu trients in solution are decreased. The growth of tobacco seedlings is also increased significantly by the fungal inocu-lation, but is inhibited generally in solution with low nutrient concentrations. The average biomasses are increased by 74. 38% (BEG168)and 48. 32% (BEG193), respectively, compared to the control(without inoculation). The phosphorus concentration in the seedlings are increased with BEGI93 inoculated, and the contents of nitrogen， phosphorus and potassium are increased with BEC168 inoculated. It is necessary to point out that the biomass and contents of phosphorus and potassium in BEG168-infected seedlings grown in nutrient solution with 75% of nutri-
收稿日期：2009-12-04
接受日期：2010-04-20
基金项目：贵州省烟草专卖局重大科技项目[2006-04]；国家烟草专卖局项目[110200401011]资助。作者简介：李建伟（1974一），男，贵州正安人，农艺师，主要从事烟草营养与载培的研充工作。
Tel; 0851 4117149, E-mail; lijianvei8996@ sina. com。万方数据
●通i作者Tel；023-68251558，E-mai：huang99@ swu.edu.cn