第36卷，第7期 2016年7月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol. 36,No.7.pp2351-2357
July,2016
CharacterizationoftheInteractionbetweenAcetvlisovalervltvlosin
TartratandBovineSerumAlbuminwithoutorwith
Zn?+ and Cu?+ by Spectroscopic Analysis
DENG Feng-yu', HU Tao-ying', ZHOU Shan-shan'-2, LIU Yingl-2
1. College of Life and Environmental Seiences , Minzu University of China , Bejing100081, China
2 . Beijing Engineering Research Center of Food Environment and Publie Health , Minzu U niversity of China , Beijing 100081 Abstract Acetylisovaleryltylosin tartrate (ATLL ) is a new macrolide veterinary antibiotic , it is necessary to study the binding of ATLL to protein , which will directly correlate with the efficiency in vivo . Bovine serum albumin (BSA) is structure homologous with human serum albumin (HSA ), and is commonly chosen as a model to investigate drug-protein interaction .There are many metal ions in plasma , as yet, the studies on mainly focus on single metal ion . In this study , the multiple systems formed by ATLL and BSA without or with Zn*+ and Cu*+ have been studied by mult-spectroscopy . The results showed that , the fluorescence of BSA was quenched by ATLL through a static quenching mechanism . The effective quenching constant (K, ) of ATLL to BSA decreased with Zn2+ and increased with Cu*+ . Thermodynamic parameters revealed that hydro -gen bonds and hydrophobie forces played significant roles in the binding of ATLL to BSA . The polarity of tryptophan and tyrosine residues changed when adding ATLL with or without Zn 2+ and Cu2+ . FT-IR spectra showed that ATLL changed αhelix and §sheet of BSA into &turn and random structure . The UV-Vis spectra indicated that the effects of Zn2+ on ATLL binding to BSA may cause by a competition binding - and Cu2+ pos-sibly formed Cu*+ -A TLL complex via metal ion bridge -. All the knowledge obtained in this work will be helpful to understand the transport mechanism of ATLL with BSA and the effect of metal ions on the interaction of drug-protein in vivo .
KeyworsAcetylisovaleryltylosin tartrat ; Bovine serum albumin ; Metal ion ; Spectroscopy
中图分类号：0657.3
Introduction
文献标识码：A
DOI ; 10., 3964/j. issn, 1000-0593(2016 )07-2351-07
in anti-bacterial and anti-mycoplasma , and has special effect on mycoplasma which is resistant to tylosin and many other antibiotics] . ATLL is also used as food additive to promote
Acetylisovaleryltylosin tartrate (ATLL ) is a new macrol-ide veterinary antibiotic , which has good effect on staphylo-coccus aureus , streptococcus pyogenes , streptococcus pneu-moniae, etc ., and overcomes the shortcoming of poor effect on pleural actinobacillusci) . Its activity is higher than tylosin
Received : 2015-06-18 ; aceepted : 2015-10-13
animal growth . However , as a new drug , to our knowledge , there are few reports on the interaction of ATLL with pro -tein . Therefore , it is necessary to study the binding of ATLL to protein , which will directly correlate with the efficiency in vivo.
Foundation item : The National Natural Science Foundation of China (21177163) , 111 Project B08044 , First-class University First Class Aca
demic Program of Minzu University of China (YLDX01013), Coordinate Development of First-Class and First-Class Univer-sity Discipline Construction Funds (10301-0150200604 ), The Academic Team Construction Project of Minzu U niversity of China (2015MDTD25C&13C), 2015MDTD08C
Biography : DENG Feng-yu , (1983), Master of College of Life and Environmental Sciences , Minzu University of China
e-mail : dengfengyu0627@ 163 .com
+ Corresponding author
e-mail , liuying4300@ 163 .com