第26卷第3期 2018年3月
1004-924X(2018)03-0541-07
文章编号
光学精密工程
Optics and Precision Engineering
便携式MicroRNA快速检测系统
王蒸飞1,2,3，余东升1,3，陈海燕1,3，张战盈1,2,3，方蔚恺1,3，杨喆1,3，
Vol.26No.3 Mar,2018
陆泽檬1-3，李艳蓄1.3，纪玉峰1.3，关一夫14，徐赤东1.3·，江海河1,2,3（1.中国科学院合肥物质科学研究院医学物理与技术中心，安徽合肥230031；
2.中国科学技术大学，安徽合肥230026；
3.医学物理与技术安徽省重点实验室，安徽合肥230031；
4.中国医科大学，辽宁沈阳110001)
摘要：MicroRNA(miRNA)与疾病的发生和发展密切相关，可作为疾病诊断标志物常规检测miRNA的方法耗时较长且操作复杂，本文设计了一种便携式miRNA快速检测系统。该系统采用光电检测技术检测滚环扩增后的标志物 miRNA受激发出的荧光强度，对采集到的荧光数据进行分析，得出miRNA的变化情况。实验得出便携式miRNA快速检测系统在试剂浓度为0.1～1μmol时，荧光强度线性偏倚不超过士5%，系统重复性大于95%，通过对冠心病检测标
志物miR-499进行滚环扩增实验，结果表明设计的便携式miRNA快速检测系统能够快速有效地检测miRNA，关键词：光电检测：荧光检测；弱光探测；miRNA；滚环扩增
中图分类号：X835
文献标识码：A
doi;10.3788/OPE.20182603.0541
PortablerapidmicroRNAdetectionsystem
WANG Yan-fei-2.3, YU Dong-shengl-3, CHEN Hai-yan'-3, ZHANG Zhan-ying'-2-3,
FANG Wei-kail-s, YANG Zhe'-", LU Ze-yuan'-3, LI Yan-leil", JI Yu-fengl-, GUAN Yi-fu'--, XU Chi-dong'-*- ,JIANG Hai-he-2..
(1.Centerof Medical Physics and Technology,Hefei Institutes of Physical Science,
ChineseAcademyofSciences,Hefei230031,China;
2.UniversityofScienceandTechnologyChina,Hefei230026,Chinq
3.AnhuiProvinceKey Laboratory of Medical Physics and Technalogy,Hefei 230031,China;
4.ChinaMedical University,Shenyang110001,China)
*Correspondingauthor,E-mailrcd@aiofm.ac.cn;hjiang@aiofm.ac.cn
Abstract: MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker. Conventional methods for the detection of miRNA are time consuming and complex. Herein, a portable rapid miRNA detection system was designed. This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique, and analyzes the fluorescence data to obtain the changes in miRNA. Through our experiments, we observed that for reagent concentrations in the range
收稿日期：2017-07-10；修订日期：2017-09-10
基金项目：国家自然科学基金资助项目（No.81371896)