研究报告
国中
酿
泉造
2012年第31卷第5期
总第242期
榆耳液态发酵培养的优化及多糖的抑菌活性
张公亮，祝明思，穆娜，刘阳，候红漫*（大连工业大学食品学院，辽宁大连116034）
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要：对榆耳进行液态发酵培养条件的优化，并考察榆耳多糖的抑菌活性。采用响应面中心复合设计方案，确定了麦芽糖(X）、豆(X,)及维生素B(X)的加入量，运用二阶多项式回归模型进行响应值预测，并进行试验验证。结果表明，榆耳液态培养最优条件为麦芽糖12.96g/L，豆柏11.68g/L，维生素B，159mg/L，配合无机盐的条件下榆耳菌丝体湿重为11.27g/100mL。多元二次回归模型为：Y= 10.50+0.75X+0.63X，+0.20X，+0.044XX，-0.075X，X，+0.088XX，-0.51X，*-0.79X，-0.55X，，因变量的总变化有92.57%可以由回归线解释。利用杯碟法考察榆耳多糖的抑菌活性，结果显示，榆耳胞内和胞外多糖对福氏志贺氏菌、枯草芽孢杆菌、鼠伤寒沙门氏菌及产气杆离有较强的抑制效果。以上结果说明，利用麦芽糖、豆柏、维生素B6配合无机盐可获得较高产量的榆耳菌丝体，其胞内多糖和胞外多糖均有一定的抑菌作用。
关键词：榆耳：液态发醇；响应面：多糖；抑菌
中图分类号：Q93-335
文献标识码：A
文章编号：0254-5071(2012)050099-05
Optimization ofliquidfermentation of GloeostereumincarnatumS.Ito et Imaiandantibacterialactivityof
its polysaccharide
ZHANG Gongliang, ZHU Mingsi, MU Na, LIU Yang, HOU Hongman*
(School ofFood Science and Technology,Dalian Polytechnic University,Dalian I16034, China)
Abstract: The liquid fermentation of Gloeostereum incarnatum S. Ito et Imai was optimized, and the antibacterial activity of polysaccharide from G. incamatum S. Ito et Imai was investigated. The doses of maltose (X,), soybean meal (X,) and vitamin B (X,) were determined by response surface methodology (RSM) and central composite rotatable design (CCRD),. A secondary polynomial regression model was constructed to determine predict-ed responses, followed by a verification test. The results showed that the optimum liquid culture conditions of G. incarnatum S. Ito et Imai were: mal-tose 12.96 g/L, soybean meal 11.68g/L and vitamin B, 159mg/L with the addition of trace inorganic salts, by which the wet weight of mycelium (Y) reached up to 11.27g/100ml. The second degree equation was Y = 10.50 + 0.75X, + 0.63X, + 0.20X, + 0.044X,X, - 0.075X,X,+ 0.088X,X, - 0.51X,2 0.79X,- 0.55X,. Regression analysis indicated that more than 92.57% of the variation could be explained by the equation. The antibacterial activity of polysaccharide from G. incarmatum S. Ito et Imai was determined by Oxford plate assay system, Experimental results showed that both intracellular and extracellular polysaccharides had high antibacterial activities against Shigella flexneri, Bacillus subtilis, Salmonella typhimurium and Aerobacter aerogenes. These results indicated that the yield of the mycelium of G. incamatum S. Ito et Imai was high on the medium containing maltose, soybean meal, vitamin B6 and mineral salts, and both intracellular and extracellular polysaccharides from G. incarmatum S. Ito et Imai had antibacterial activi-ties.
Key words: Gloeostereum incarnatum S. Ito et Imai; liquid fermentation; response surface methodology; polysaccharide; antibacterial activity
榆耳（GloeostereumincarnatumItoetImai)又称榆蘑，是一种珍贵的药食两用真菌，其子实体营养丰富，含有蛋
收稿日期：2012-02-12
基金项目：大连市工程技术研究中心项目(20090436）
白质、碳水化合物、多种维生素以及钙、磷、铁等矿物质元素"。目前，榆耳人工栽培品种都是由野生种别化得，产量
作者简介：张公亮(1978-)，男，讲师，研究方向为天然活性物质和食品安全检测：候红漫*，教授，通讯作者。
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