中圃届复医学集志2011年，第26卷.第2期
·基础研究·
运动训练对脊髓损伤大鼠Nogo-A、NgR mRNA 表达的影响
周治来
摘要
陈银海2.3
新安民！
瘾2 刘
目的：探讨运动训练对脊髓损伤(SCI)大鼠髓鞘源性神经抑制因子(Nogo-A)及其受体(NgR)mRNA表达的影响，
方法：44只SD大鼠随机分为运动训练组16只、损伤组16只及假手术组12只，采用改良Allens打击法制作大鼠 T10脊髓损伤模型。造模后每周应用BBB评分法观察大鼠后肢运动功能，在第810、14天处死大鼠，每组4只，以荧光定量PCR法测定不i点脊髓组织中Nogo=A与NgRmRNA相对含量：
结果：手术后第4—8M.is动训练组大鼠BBB评分明显高于SCI组；运动训练组与SCI组Nogo-A与NgRmRNA表第8、10天，SCI组Nogo=AmRNA表达均显著高于运动训练组与假手术组（P<0.05），至第14天，运动训练组与SCI 组表达差异无显著性意义（P>0.05），但均高于假手术组(P<0.05)，假手术组在各时间点表达差异均无显著性意义(P> 0.05);SCI组在各时间点NgRmRNA表达均高于运动训练组与假手术组（P<0.05）.运动训练组在第10天便下降至与假手术组差异无显著性意Z:P>0.05).假手术组在各时间点表达无差异(P>0.05)。
结论：康复训练能减少Nogo-1.>RmRNA的表达，促进脊髓损伤大鼠后肢功能的恢复，关键词
脊髓损伤；康复训的：：>ugu-A；NgR；BBB评分
中图分类号：R651.2.R493
文献标识码：A
文章编号：10011242(2011)-020143-05
Zhilai, CHEN Yinhai, JIN Anmin, et al.J/Chinese Journal of Rehabilitation Medicine, 2011, 26(2): 143147 Abstract
Objective:To explore the effects of exercise training on the expression of NogoA and its receptor NgR in spinal cord injury(SCI) rats.
eadesneseaeaeandoo group 12 rats)randomly. Rats of test group experienced modified Allen impactor (H=25mm)at T10, then were ran-domly divided into 2 groups equally: trainingtreated group and SCI group. The hindlimbs movement was assessed by BassoBeattieBresnahan(BBB) scale and the injured spinal cord tissue was extracted for NogoA and NgR mR-
NA examination through SYBR Green realtime PCR on the 8th,1Oth.14th day after SCI. Result:Compared with SCI group.the BBB scol
exercise trainingtreated group showed marked improvement be-
tween the 4th week and the 8th week after injury NogoA and NgR mRNA in trainingtreated group declined gradually as time prolonged.
that in training-treated grouy
and shar
no significant difference between SCI group
DOI:10.3969/j.issn.10011242.2011.02.013
( P<0.05),Compared with shamoperated group,the expressions of
increased significantly postoperation(P<0.05 ),then
group
andtraining-treated
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significantly higher than was
rato
(P<0.05), there was
14th day, but all higher than that in the
1南方医科大学珠江医院骨科中心，广州，510282：2南方医科大学球江医院康复医学科；3
讯作者
作者简介：周治来，男，硕士研究生：收稿日期；2010-01-24
www.rehabi.com.cn
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