现代肿瘤医学2010年05月第18卷第05期
肉瘤化疗疗效和预后的指标。在探索判断骨肉瘤化疗疗效及预后指标方面，Ki-67仍是最有希望的标志物，但在骨肉赠中的作用这一历程中，仍需加大样本含量并获得有效的患者随访资料进一步研究探讨。
【参考文献】
[1] Guo W,Yang R,Tang X,et al. Neoadjuvant chemotherapy for ostro-
sarooma[J].Natl Med J China,2004,84(14) :1186 1190[2]
蔡栖伯，牛晓辉，张清，等.肢体原发成骨肉瘤综合治疗的远期结果[刀].中华外科杂志，2000,38(5)：329-331.
Mika MS,Maija T, Aame HK,et al. Osteosarcoma in Finland from[3]
1971 through 1990 A nationwide study of epidemiology and out-come[J].Acta Orthopaedica,2008,79 (6) :861 866
樊祥山，孟凡青，章宜芬，等，骨肉瘤组织芯片的制作及其免疫[4]
组织化学应用[J].东南大学学报（医学版），2006，25（2)：104 106.
[S]
Ahmed A, Gilbert Bamess E, Lacson A. Expression of c kit in Ewing family of tumors: a comparison of different immunohisto-chemical protoeols[J]. Pediatr Dey Pathol, 2004,7 (4) : 342 347.
[6] de Alava E, Antonescu CR, Panizo A,et al. Prognostic impact of
P53 status in Ewing sarooma[J]. Canoer, 2000,89 (4) : 783 792.
张春林，离威明，李佛保，等，骨肉瘤P2IWAFI蛋白、PCNA与[7]
Ki-67的表达及其意义[J].中国癌症杂志，2001,11(2)：109
[8]
-112.
·983·
于秀淳，徐明.新辅助化疗对B期骨肉瘤细胞CyelinD1、Bel-2、PCNA和P-gP表达的影响[J].中华肿瘤防治杂志，2006， 13(23):18011804
[9] Siller CA,Passmore SJ, Kroll ME,et al. Pattems of care and sur-
vival for patients aged under 40 years with bone sarcoma in Britain, 1980 1994[J].Br J Cancer,2006,94(1) :22 29.
Zuffa E,Mancini M,Brusa G,et al. P53 oncosuppressor influences
[10]
selection of genomic imbalances in response to ionizing radiations in human osteosarcoma cell line SAOS 2[J], Int J Radiat Biol, 2008.84(7):591601.
[n]
Lengner CJ,Steinman HA, Gagnon J, et al. Osteoblast differentia tion and skeletal development are regulated by Mdm2 p53 signa-ling[ J]. J Cell Biol,2006,172(6) :909 921.
Lonardo F,Ueda T, Huvos AG,et al. p53 and MDM2 alterations in
[12]
osteosarcomas: corelation with clinioopathologic features and pro-liferative rate[J].Cancer,1997,79(8) :1541 1547.
Gokgoz N,Wunder JS,Mousses S,et al. Comparison of p53 muta-
[13]
tions in patients with localized osteosarcoma and metastatic osteo-sarcoma[J].Canoer,2001,92(8) :2181 2189
Kaseta MK,Khaldi L, Gomatos IP,et al. Prognostic value of bax
[14]
bel 2,and p53 staining in primary osteosarooma[J].J Surg On-eol,2008,97(3):259 266.
（编校：李鹏超）
血小板反应蛋白-1的表达与骨肉瘤新生血管生成的研究宋玉鑫”，刘守正”，张辉”，胡红霞，段大鹏”，王建民
Theresearchto expressionofthrombospodin-1andangiogenesisofOsteosarcoma SONG Yu - xin',LIU Shou -zheng",ZHANG Hui',HU Hong -xia',DUAN Da -peng", WANG Jian - min
'Peoples Hospital of Gansu Protince,Lanchou 730000,China;"People3 Hospital of Shuyang,Jiangsu Shuyang 223600,China;'People3 Hospital of Shaanxi Prouince,Xian 710068,China;"The First Hospital of Lanzhou University,Lanzhou 730000,China.
[Abstract] Objective:To explore the expression of thrombospodin 1(TSP 1) in osteosarcoma and investigate its relationship with tumor angiogenesis, Methods: The expression of TSP 1 was evaluated by immunohistochemical staining on tumor specimens from 54 patients with osteosarcoma, The microvessel density( MVD) was assessed by im-munostaining with CD34 in the most eovascularized area (hot spots). Results; In osteosarcoma,the mean microves-sel count of TSP 1 high expression tumors was significantly lower than that of TSP -1 Jow - expression tumors. The close correlation was found between TSP 1 expression and microvessel density. TSP 1 expression and microvessel density was negatively correlated with lung metastasis in osteosarcoma. In contrast,no association was found between TSP1 expression and gender,age,size and location of tumor,osteosarcoma classification,as well as microvessel
【收稿日期】【修回日期】【作者单位】
20090716 20090915
1甘肃省人民医院，甘肃
兰州730000
沐阳县人民医院，江苏
沐阳
"陕西省人民医院，陕西
西安
*兰州大学第一医院，甘肃
223600 710068
兰州
730000
宋玉鑫（1981-），男，山东滩坊人，硕士，医师，主要从事骨与关节疾病、骨肿瘤的临床诊疗工作。E-mail：songyuxin-1@163
【作者简介】
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