第35卷，第12期 2015年12
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol.35,No.12-pp3560-3564
December, 2015
EffectofREAuonSilverStainingResultsand
Their UV-Vis Absorption Spectra
LIU Xiao-zhen',FENG Jun-liang',LIU Xiao-zhou
CHEN Jie",ZHU Wan-wan',GUIXiu-min
1. School of Chemical and Environmental Engineering , Shanghai Institute of Technology , Shanghai 201418 , China
2. Gannan Normal University , Ganzhou 341000 , China 3. Regenia AB, Stockholm10691, Sweden
Abstract La, Ce , Nd, Sm , Eu, Gd, Dy particles and gold nanoparticles were prepared . Effects of La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au and Dy-Au particles on silver staining results were studied , respec-tively , and UV-Vis absorption spectra of La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au and Dy-Au parti-cles were studied. Times and colors of sports with La-Au, Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au, Dy-Au particles are longer and darker than that of with gold nanoparticles , respectively . The time of sport with Nd-Au particles is as long as 30 min , which is 2. 7 times as long as with gold nanoparticles . Although amount of gold nanoparticles reduced 80% , the eolor of sport with Nd-Au particles is darker than that of with gold nano-particles . In 200, 00800. 00 nm, La , Ce, Nd, Sm, Eu, Gd , Dy particles and gold nanoparticles has one ab-sorption peak , respectively , and Arx is 275 , 277 , 276 , 276 , 278 , 277, 278 and 521 nm , respectively . La-Au , Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au, Dy-Au particles have two absorption peaks , respectively , Asax(RE) and Anx(Ae) are276 and 522 nm,276 and 522 nm,276 and 523 nm,276 and 523 nm,276 and522 nm,276 and 522 nm , 276 and 523 nm , respectively . Auα of Au nanoparticles and La particles occurs red moving respective ly , and Au of Ce , Eu, Gd and Dy particles occurs blue moving + respectively + and An of Nd and Sm particles
is constant respectively . Rare earths particles and gold nanoparticles may have interaction , respectively -Keywords Gold nanoparticles ; Rare earths ; Silver staining ; U V-Vis absorption spectrum
中图分类号：0657.3
Introduction
文献标识码：A
DOI: 10, 3964 /j. issn. 1000-0593(2015 )12-3560-05
the price of gold is expensive . Replacement of gold with chea-per metal is the current research focus . Rare earths have been widely used in optics, electronics , metallurgy , chemical and
Methods of gene and protein detection in combination with nanotechnology + particularly those techniques involving gold nanoparticles , have become increasingly in biological re-search-12] . Methods of gene and protein detection by "gold label silver staining" technology have been one of the most rapidly developed methods'] . Gold is a precious metal , and
Received : 2014-08-05 ; aceepted : 2014-12-20
materials engineering , due to their special physical and chemi-cal characteristicscia, 14] . In this paper, La, Ce, Nd, Sm , Eu Gd , Dy particles and gold nanoparticles were prepared respec tively . Replaced part of gold nanoparticles with La , Ce, Nd, Sm , Eu, Gd , Dy particles respectively , and effects of La-Au , Ce-Au, Nd-Au, Sm-Au, Eu-Au, Gd-Au, Dy-Au particles on
Foundation item: the National Natural Science Foundation of China (51172144 , 51372153 ), Science Technology Foundation of shanghai
(12410710300 ), Leading Academic Discipline Project of Shanghai Municipal Education Commission (J51503 ) and the Pro-gram for Professor of Special Appointment (Eastern Scholar ) at Shanghai Institutions of Higher Learning
Biography : LIU Xiao-zhen , female , (1958—), Professor of School of Chemical and Environmental Engineering , Shanghai Institute of Technol-
seys80
e-mail : liuxiaozhen1958@ yahoo -com