植物营养与肥料学报2017,23(5):1314-1325 Journal of Plant Nutrition and Fertilizer
doi: 10.11674/zwyf.16451 http:/www.plantnutrifert.org
不同硝铵比氮素供应对广香生长及药效成分的影响
卢丽兰"，杨新全'，王彩霞2，符式龙"，梁振益3
（1中国医学科学院药用植物研究所海南分所，海南海口570100；2海南省农业科学院，海南海口570100：
3海南大学，海南海口570228）
广蕾香的产量和药用品质提供氮肥施用依据。【方法】以海南藿香为研究材料进行了砂培盆栽试验。设5个不同NO,-N/NH,-N比处理为100:0、75：25、50：50、25：75、0：100，每隔10天浇一次，每次250mL/盆，每盆定植1株6cm高的广蕾香组培苗。采收期分为定植后60、120、180、240天，每个处理每次采取4盆，分
【结果】硝铵比为75：25的处理更能促进广藿香的株
析了广蓄香生长指标、叶绿素、抗氧化活性及药效成分。
高、茎粗、分兼数、叶面积指数、地上部鲜重、
搬上部重
茎含油率、叶含油率、全株含油率和单株含油量
增加，地有利于叶绿素a和叶绿素a+b含量提高。
、殖铵比为7
5处理的广蕾香植株N、P、K、Ca、Mg元
素吸收最多，硝铵比为25：75和0：100处理的较低。硝铵比为50：50的处理有利于叶绿素b含量和苯丙氢酸解氨醛（PAL酶）活性的提高，有助于广蕾香茎叶的广著香酮的提高。硝铵比为25：75和50：50的处理更有利于羟自由基、超氧阴离子自由基、DPPH自由基清除率的提高。硝铵比25：75处理有利于广蓄香茎叶广蕾香醇和其他主要药效成分含量的提高。全硝态氮和全铵态氮（硝铵比为100：0和0：100）处理不利于或抑制广藿香茎叶油的药效成分形成和积累。【结论】供应硝铵比为75：25的氮更能促进广蓄香生长和光合效能的提高，硝
铵比为50：50、25：75处理更能促进广蓄香主要药效成分含量的提高。关键词：广蓄香；氨形态；生物量；药效成分
Effects of nitrogen supply with different NO,/NH, ratios on growth and
medicinalcomponentsofPogostemoncablin
LU Li-lan', YANG Xin-quan', WANG Cai-xia', FU Shi-long', LIANG Zhen-yi
( I Hainan Branch of Institute of Medicinal Planr Development, Chinese Academy of Medical Sciences, Haikou 570100, China,
2 Hainan Academy of Agricaltural Sciences, Haikou 570/00, China; 3 Hainan University, Haikou 570228, China )
Abstract: [ Objectives ] The aim of this paper was to study the suitable supply ratio of NO,/NH, for high yield and contents of medicinal functional composition in Pogostemon cablin.[ Methods] The 6 cm tissue culture seedlings of Hainan Pogostemon cablin were taken as the research material in a sand culture experiment. Five ratios of NO,-N/NH,-N were set up: 100 : 0, 75 : 25, 50 : 50, 25 : 75 and 0 : 100. Each pot was planted with 1 seedling and irrigated with 250 mL of N solution each time and at 10 day intervals. The plant samples were collected 60, 120, 180 and 240 days after the transplanting. The patchouli growth indexes, chlorophyll composition and antioxidant activity and efficacy were analyzed. [ Results ] The NO,-N/NH,'-N ratio of 75 : 25 promoted plant height, basal culm thickness, tiller number, leaf area index, above-ground fresh and dry biomass, shoot oil rate, leaf oil rate, whole plant oil rate and plant oil amount, and also improved the contents of chlorophyll a and chlorophyll (a + b). The NO,-N/NH,*-N ratio of 75 : 25 was better for the absorption of N, P, K, Ca and Mg; the ratio of 50 : 50 was beneficial to improve the content of chlorophyll b; the ratios of 25 : 75 and 50 : 50 were more conducive to improve the free radical scavenging rate of hydroxyl, superoxide anion and DPPH; the
收稿日期：2016-11-30
接受日期：2017-03-31
基金项目：海南省重大科技项目（ZDZX2013008-1）资助。
作者简介：卢丽兰（1983一），女，海南海口人，博土，副研究员。主要从事土壤肥料，植物营养及植物化学方面的研究。
E-mail;lulilan1234@163.com。*通讯作者E-mail;yangxinquan@sina.com
万方数据