植物营养与肥料学报2010,16(3)：701-707 Plant Nutrition and Fertilizer Science
渭北旱塬葡萄园土著AM真菌对赤霞珠葡萄
促生效应研究
房玉林12，张稼涵"，宋士任"，屈雁朋"，张振文1,2，孟江飞"，刘金串1，孙艳！（1西北农林科技大学葡莓酒学院，陕西杨凌712100；2陕西省葡费与衡都酒工程技术研究中心，陕西杨凌712100）
摘要：以筛选白滑北早源葡翻网的3种土著AM真菌菌株Glomusmosseae（CM）、Glomusintraradices（CI）和Glomuster-siforme(CV)为试材，在盆栽条件下研究了3种AM真菌单独接种及混合接种对酿酒葡静亦霞珠（CabermetSauvignon）
接种；混合接种CI+CV>GM+GV>CM+CI,单接种GV>GI>GM。接种AM真菌促进葡葡幼苗的生长，显著提高植株高度和地上、地下干重，并提高葡萄叶片净光合速率、气孔导度、胞间二氧化碳浓度和蒸聘速率，从面提高葡萄叶片的光合作用；葡能叶片中叶绿素含量、可落性糖含量、可溶性蛋白含量均高于未接种对照，且混合接种优于单接种。
关键词：葡毒；AM真菌；促生效应
中图分类号：S663.1
文献标识码：A
文章编号：1008-505X(2010)03-0701-07
Promotioneffectsof nativeAMfungion CabernetSauvignongrapevines
in vineyards of Weibei Tableland
Ns*uer a ONuan NHZ uad O uars ONO ONVH N
(I College of Enology, Northauest A & F Unitersiy, Yangling, Shaanxi 712100, China;
2 Shaanzi Engineering Research Center for Viti-tiniculure, Northwest A & F University, Yangling, Shaanxi 712100, China)
Abstract:The growth promoting effects of three native AM fungi (Glomus mosseae, GM; Glomus intraradices, GI; Glo-mus wersjforme, GV) which were isolated from vineyards in Weibei Tableland on Cabemet Sauvignon grapevines seedlings were investigated by using the controlled conditions with different inoculation treatments. Arbuscular mycorhizae were found to form in the grapevine roots afer inoculated with AMF, and there were differences of colonization among the dif-ferent inoculation treatments. The results show that the mixed-fungi treatments have more mycorrhizal infection rates than those of the single fungus treatments. The mycorrhizal colonization caused by inoculum of CI + GV is of the largest effi-cient , followed by GM + GV, the least is GM + GI. When inoculated with single fungus, the mycorrhizal infection rate as follows: GV > GI > GM. At the same time, the plant height and dry weight are significantly increased. The inoculations of AM fungi can significantly increase Pn, Gs, Ci and Tr, and thereby enhance the photosynthesis of grape leaves, and the mixed-fungi inoculations have better effects than those of the single ones. Furthermore, the inoculations with AM fun-
gi can significantly increase the contents of chlorophyll, soluble sugar and soluble protein of grape leaves Key words: grapevine; AM fungi; promotion effects
丛枝菌根真菌（Arbuscularmycorhizafungi，AMF）是自然界中分布最广的内生菌根，它能与自然界中约有90%的维管植物共生形成丛枝菌根1-3]。AM
收稿日期：2009-06-30
接受日期：2009102
真菌可以直接影响包括植物的矿质营养吸收、生长发育[2-4]及抗逆性[5-7]、抗病性[8-10],提高移裁成活率等(3-5)；并通过对土壤结构及生物群落结构的影响
基金项目：现代农业产业技术体系建设专项资金；陕西省"13115"计划项目；西北农林科技大学青年学术骨干支持计划资助。作者简介：房长林（1973一），男，河南兰考人，博士，副教授，主要从事葡荐裁培、生理生态研究。
Tel: 02987091994, E-mail: fangyulin@ nwsusf.edu.en 万方数据