植物营养与肥料学报2010,16(3)：708-713 Plant Nutrition and Fertilizer Science
高寒地区联合固氮菌肥对青稞的促生效应研究
张1,2，姚拓1*，张德罡1，辛国荣2
（1甘肃农业大学草业学院，甘肃兰州730070；
2有害生物控制与生物资源利用国家重点实验室，中山大学生命科学学院，广东广州510275）
摘要：在高寒地区，通过裂区试验研究联合周氮菌菌肥对青稞生长的影响。结果表明，本试验研制的联合固氮菌肥符合《农用微生物菌剂》质基标准（GB20287-2006)。联合固氮菌肥对青稞主根长度（乳熟期）、地下生物量（完熟期)、粗蛋白含量(抽穗期、灌浆期和乳熟期）、子粒产量均有显著的促进作用。其中，固氮菌肥+半氮肥(1/2CN) 处理，对于株高（苗期）、地上生物量（滞浆期、乳熟期和完熟期）、主根长度（乳熟期）、根体积（激浆期）、地下生物量(乳熟期和完熟期）、粗蛋白含量（整个生育期）、子粒产量等与对照相比差异显著（P<0.05)；面与全录氮肥(CN)处理相比，其株高（抽穗期、灌浆期和乳熟期）、叶绿求含基（整个生育期）、地上生物量（抽穗期和乳熟期）、主根长度（蒲浆期和完熟期）、根体积（抽穗期、蒲浆期和完熟期）、地下生物量（警个生育期）、粗蛋白含量（完熟期）虽低于CN 处理，但两者间并无显著差异。说明在一定生育期内，半景氮肥+周氮菌肥(1/2CN)处理表现出了与全量氮肥相近
的促生效果，即在该地区以"联合固氮菌肥+半量氮肥"的施用方式可以节省一半氮肥(56.25kg/hm")。关键词：高寒地区；青稞；联合周氮菌肥；促生效应
中图分类号：S512.3；S144.5
文献标识码：A
文章编号：1008505X(2010)03-0708-06
Effects of associative nitrogen-fixing biofertilizer on growth of
Hordeumvulgarein alpineregion
ZHANC Kun'-2, YAO Tuol'* , ZHANC De-gang', XIN Guo-rong
(I Pratacultural College, Gansu Agricultural Unitersily, Lanzhou, Gansu 730070, Chine;
2 Stale Key Laboratory for Biocontrol, School of Lijfe Sciences, Sun Yat-sen Unitersily, Guangzhou, Guangdong 510275, Chine)
Abstract: The effects of bio-fertilizers on the growth of highland barley ( Hordeum tulgare ) in alpine regions were studied by using the split-block design experiment. The bio-fertilizers were composited with the associative nitrogen-fixing bacte ria ( Pseudomonas sp., Azotobacter sp., Zoogloea sp.). The results indicate that the associative nitrogen-fixing bio-fer-tilizers accord with the quality criterion of agricultural microbial fertilizer of China (GB 202872006). The root length(dough stage), underground plant biomass (mature stage), crude protein (heading stage, postulation stage and dough stage) and grain yield are significantly promoted by the bio-fertilizer applications ( P < 0.05). In the 1/2CN treatment group (bio-fertilizer + half application rate of chemical nitrogen fertilizer), the height (seedling stage), aboveground biomass (postulation stage, dough stage and mature stage), root length (dough stage), root volume (postulation stage), underground biomass (dough stage and mature stage), crude protein (whole stage) and grain yield are significantly high-er than those of the control ( P < 0.05), The plant height (heading stage, postulation stage and dough stage), chloro-phyll content (the whole stage), aboveground biomass (heading stage and dough stage), root length (postulation stage and mature stage), root volume (heading stage, postulation stage and mature stage), underground biomass (the whole stage), crude protein (mature stage) of the 1/2CN treatment are lower than those of the CN treatment (the whole appli-
收稿日期：2009-0615
接受日期：2009-11-02
基全项目：国家自然科学基金(30960265)；农业部熊变产业体系和现代农业产业技术体系建设专项资金资助。
作者简介：张垫（1979—），男，甘肃天水人，博让研究生，主要从事草地农业生态研究。Tel：020-84112008，E-mail：gchangkun@163.eom
通讯作者Tel:09317631227，E-mail:yaotuo@gau.edu.en
试验中得到徐长林副研究员、席端乔研士，韩文星硕士的帮助，特致谢忧。万方数据