第36卷，第9期 2016年9月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol.36,No.9+pp3053-3061
September, 2016
Research onMolecular Spectra ofInteractionsBetween SalvianolicAcidAandSalvianolicAcidBwithInsulin:
and Effect of Glucose on the Binding
YANG Wen-yue',CUI Lin',QU Ling-ling',XU Ning',HUANGYunl-2+
CUILi-jian*,ZHANWen-hong',ZHAO Ding
1. Pharmaceutical College, Hebei Medical University, Shijiazhuang 050017, China
2. Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang050017, China 3. Experimental Center, Hebei University of Chinese Medicine, Shijiazhuang 050091, China
Abstract
The interactions of Salvianolic acid A (SAA) and Salvianolic acid B (SAB) with insulin were studied
by using fluorescence spectroscopy, UV-vis spectroscopy and ATR-FTIR spectroscopy in simulating physio-logical condition (pH 7. 40). The fluorescence quenching of insulin by SAA and SAB were static quenching process, The results of synchronous fluorescence and three-dimensional fluorescence spectra suggested no ob vious conformation changes of insulin after SAA or SAB binding. But ATR-FTIR spectra showed that SAA and SAB could change the secondary structures of insulin, of which β-turns decreased and random coil in-creased accompanied with α-helices and β-sheets no clear change, The glucose might influenced the the bioactiv-ity of insulin in the SAA-insulin and SAB-insulin systems by changing the binding constants of SAA (or SAB)
with insulin and exacerbating the changes of insulin conformation and relative contents of α-helices. Kevwords
Salvianolic acid A; Salvianolic acid B; Insulin; Glucose; Spectroscopy
中图分类号：0446.1
Introduction
文献标识码：A
DOI: 10. 3964/j. issn. 10000593(2016)093053-09
pounds[5-9) in DANSHEN injection.
Diabetes mellitus is a serious pathological disease espe cially with various acute or chronic complications such as dia
Danshen injection is a kind of traditional Chinese medi-cine (TCM) injections often used in clinical for the treatment of diabete complications including peripheral neuropathy and microangiopathy.1-2). Some researchs show that water-soluble phenolic acids compounds could prevent diabetic nephropathy, due to improving abnormal blood rheology of diabetes mellitus patients[3-. Salvianolic acid A (SAA) which was demonstra-ted to have the function of cardiovascular protection and Salvi-anolic acid B (SAB) which was used in clinical practice for the treatments of myocardial infarction and coronary heart disease(Fig,1) are important water-soluble phenolic acid com-
Received: 2015-05-06 ; aceepted: 2015-09-17
betic nephropathy, diabetic cardiopathy, and diabetic enceph-alopathy, which usually need intravenous therapy. But lots of normal saline (NS) infusion is liable to cause water-sodium retention and homeostasis disturbances when drugs are given via a peripheral venous access. In clinical, these patients are given drugs and insulin dispensed in 5% glucose injection through intravenous drip instead of NS[to).
Insulin plays important roles in blood glucose, influences lipid metabolism,protein degradationsynthesis,and growth, which is a polypeptide hormones secreted by pancre atic β cell. It is composed of A peptide chain (21 amino acids)
Foundation item: the HEBEI Province Science and Technology Support Program (142777114D), College Students' Innovative Entrepreneurial
Training Programs (USIP201525A)
Biography : YANG Wen-yue, (1989—), female, postgraduate student, Pharmaceutical College of Hebei Medical University
Corresponding authors
e-mail: huangyun9317@126. com; cuilijianzy@126. com