植物营养与肥料学报2014,20(2）：280-289 Journal of Plant Nutrition and Fertilizer
doi=10.11674/zwyf.2014.0203
氮肥水平对玉米灌浆期穗位叶光合功能的影响
王帅，韩晓日*，战秀梅，杨劲峰，王月，刘轶飞，李娜
（沈阳农业大学土地与环境学院，土壤肥料资源高效利用国家工程实验室，
农业部东北玉米营养与施肥科学观测试验站，辽宁沈阳110866）
摘要：本文在棕壤长期定位实验的基础上，测定了不同水平氮肥处理下灌浆期玉米（郑单958）穗位叶气体交换和叶绿素荧光等光合参数以及SPAD值。结果表明，施用氮肥可以引起荧光诱导曲线（OJIP曲线）显著变化，其中K、 J和I点的荧光强度值明显降低：施用氮肥显著提高捕获的激子将电子传递到电子传递链中Q，下游的其他电子受体的概率（亚。）和以吸收光能为基础的性能指数（PIas），显著降低K点的可变荧光F，占振幅F。F：的比例（W，)和 J点的可变荧光F，占振幅F。F，的比例（V）说明PSIⅡ反应中心电子传递链综合性能以及供体侧和受体侧的电子传递能力均明显提高，并且可以看出受体侧性能的改善大于供体侧：随着施氮量的增加，PSI的最大氧化还原活性 AI/I。呈先升后降的趋势变化，说明适量施氮可以增强Pra的最大氧化还原能力，并可以显著提高叶片PSI与PSⅡ 两个光系统之间的协调性中(rsI/rsI)：
关键词：玉米；氮肥；光合系统；叶绿素荧光；820nm光吸收
中图分类号：S14-35；S141.4*4；S513
文献标识码：A
文章编号：1008-505X(2014)02-0280-10
Effectof nitrogenousfertilizerlevels onphotosyntheticfunctions
of maize ear leaves at grain filling stage
WANG Shuai, HAN Xiao-ri*, ZHAN Xiu-mei, YANG Jin-feng, WANG Yue, LIU Yi-fei, LI Na
( College of Land and Enironment, Shenyang Agricultural University/National Engineering Laboratory of High Efficient Use
on Soil and Fertilizer Resources/Experiment Station of Northeast Maize Nutrition and Fertilization,
Ministry of Agriculture,Shenyang,Liaoning I10866,China)
Abstract: On the basis of a long-term experiment in brown soil, the photosynthetic characteristics of maize(cultivar, Zhengdan 958) ear leaves at the filling stage , SPAD value, gas exchange and chlorophyll fluorescence parameters were measured under different levels of nitrogen treatments . The results show that the OJIP Fluorescence induction curve is affected significantly by nitrogen fertilization , and the fluorescence intensity of K , J and I steps are significantly decreased. The probability that a trapped exciton moves an electron into the electron transport chain beyond Qa (,) and the performance index on absorption basis (PIaes ) are significantly increased under the nitrogen fertilization, while the relative variable fluorescence intensity at the K -step ( W) and the relative variable fluorescence intensity at the J-step (V,) are significantly reduced. It is shown that the comprehensive performance of electron transport chain in PS Il reaction center as well as the electron transport capacity both in the donor side and the receptor side are improved obviously . The performance of the receptor side is greater than that of the donor side. With nitrogen fertilizer levels increasing, the maximal oxidation-reduction activity of Ps I（AI/I。） is increased first and then decreased , which indicates a moderate amount of nitrogen fertilizer could enhance the maximal oxidation-reduction ability of Proo , and could significantly improve coordination between PS I and PS II optical systems (rs I /ps ) in maize leaves.
Key words maizenitrogen fertilization photosynthetic systemchlorophyl fluorescence 820nm absorbance
收稿日期：2013-05-13
接受日期：2013-10-20
基金项目：国家自然科学基金（31071861）：国家科技支撑计划课题（2011BAD16B12）资助。
作者简介：王帅（1982—），男，辽宁铁岭人，博士，从事植物营养与施肥研究。E-mail：wangshuai080982@163.eom
*通信作者E-mail：hanxiaori@163.eom