植物营养与肥料学报2010,16(3)：536-542 Plant Nutrition and Fertilizer Science
氮素对玉米灌浆期叶片光合性能的影响
李耕"，高辉远2.3，刘鹏1.3*，杨吉顺"，董树亭1,3，张吉旺1,3，王敬锋1
（1山东农业大学农学院，山东泰安271018；2山东农业大学生命科学学院，山东泰安271018：
3作物生物学国家重点实验室，山东泰安271018)
摘要：以玉米自交系齐319(Q319)为材料，采用单株盘赖种植方式，借助叶绿素荧光快速诱导动力学曲线和820nm 光吸收曲线，研究了氮索对玉米灌浆期叶片光合性能的影响。2年研究结果表明，在本试验条件下，氮求对Q319灌浆期叶片叶绿索含景无明显提高作用，但其净光合速率(P,)和单株干物质积累量、子粒产量显著增加。JIP-test分析表明，氮素显著提高了叶片光系统IⅡ（PSⅡ）反应中心电子供体侧和受体侧性能：显著提高了Q319PSⅡ反应中心电子受体侧之后的电子传递链性能，增强了电子由PSⅡ向PS1的分配，从面显著地提高了PSⅡ与PSI之间的协调性。可以认为，施氮后藩浆期叶片叶绿索含的变化不是P，提高的主要原因，而两个光系统性能的改善及二者间
协调性的提高增强了光合电子传递链的性能是灌浆期P，升高与成熟期产量增加的主要原因。关键调：氮索；玉米；灌浆期；光合性能；光系统
中图分类号：S513.06
文献标识码：A
文章编号：1008505X(2010)030536-07
Effectsof nitrogenfertilizationonphotosyntheticperformance
inmaize leaf at grain filling stage
LI Geng", GAO Hui-yuan2-3, LIU Peng.-3 ·, YANG Ji-shun', DONG Shu-ting'-3, ZHANC Ji-wang'-3, WANG Jing-feng'(1 College of Agronomy, Shandong Agriculrural Uninersity, Tai' an 271018, China; 2 College of Lijfe Science, Shandong Agriculura
Uninersily, Tai'an 271018, China; 3 Sate Key Laboralory of Crop Biology, Tai'an 271018, China)
Abstract: A pot experiment was carried out to study the effects of nitrogen fertilization on photosynthetic performances in maize leaves of inbreed line Qi319 (Q319) at grain filling stage using simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm. Two years' results indicate that N fertilization had no effect on chlorophyll con-tents in the ear leaf at grain flling stage, but significantly improved the net photosynthetic rate( P,) and single plant dr) matter and grain yield ( P < 0.05) of Q319. JIP test analyzing showed that the nitrogen fertilization significantly increased the performances of electron donor and acceptor in photosystem II (PS I ) reaction center ( P < 0.05). Fur-thermore, nitrogen fertilization significantly increased extent of performance on electron transport chain after acceptor in Q319 PS II reaction center was significant, and enhanced the distribution of electron transport from PS II to PS I . Con-sequently, the coordination between PSI and PSI was significantly improved. We considered that, under nitrogen fer-tilization treatment, the increasing of P, and yield was not mainly resulted from the change of chlorophyll content at grain filling stage, but from the enhancement in Q319 photosynthetic performances and improvement of the coordination between PS II and PS I and performance of electron transport chain.
Key words; nitrogen; maize; grain filling stage; photosynthetic performance; photosystem
氮素作为蛋白质(酶)及核酸的重要组成元素，对器官建成、根冠发育、光合作用、碳、氮关系、源库
收稿日期：2009-08-19
接受日期：2009-12-16
关系等有全面的影响]。缺氮会抑制作物生长和光合作用，是造成产量和品质下降的重要原因[2-3]。
基金项目：国家科技支撑计划项目（2006BAD02A13-2-2，2007BAD91B04)；国家自然科学基金项目（30871476）；山东省良种工程项日资助。作者简介：李耕（1981—），男，山东济南人，硕士，主要从事玉来载培生理生态研究。Tel：0538-8245838；E-mail：ligeng213@163.com
通讯作者Tel:05388245838;E-mail; liupengsdau@126.com
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