植物营养与肥料学报2017,23(2)：434443 Jourmal ofPlantNutritionandFertilizer
doi: 10.11674/zwyf.16239 http://www.plantnutrifert.org
氮硫互作提高大蒜氮、硫含量及其关键同化酶活性
许建"2，贾凯，朱君芳，胡梅"，王娜，高杰1*
（1新疆农业大学林学与园艺学院，乌鲁木齐830052；2新疆农业职业技术学院园林科技学院，新疆昌吉831100）摘要：【目的】从生理学角度研究氮、硫两种营养元索配施对大幕氮硫关键同化酶的影响，揭示氮硫关键同化酶与植株氮、硫同化能力的关系，以期为大薪合理施肥与提质增效提供理论参考。【方法】采用蛭石-珍珠岩
盆裁方式，研究了不同浓度氮(5、10、20mmol/L）
长期、鳞茎膨大初期和中期大蒜植株氮风含量总体呈上升趋势，在解茎膨大期运
先上升后下降趋势，在花茎(ATPS)活性在花蒸伸长
期酶活性总体最低大期，单因索影响显著，而对GS影
NR、GS均有显著或机作用对其影响极显著，
长期、鳞基膨大初期与植株硫含量
影大初期和中期，GS活性与氮含量呈显著负相关
体呈负相关，而在鳞茎膨大初期为0.646，呈显著正相关。
8mmol/L)配施条件下，大蒜在幼苗期、花茎伸
化酶活性的动态变化。【结果】大蒜植株
司售
谷氨酰胺合成酶(GS)活性变化呈
后平稳趋势
ATP-硫酸化酸
上升趋势，
在花茎伸长
因素影响不明显；鳞茎膨
鼠素对于NR活性影响而氮硫交互作用对
现案
相天关系
，而氮硫交互
ATPS活性在花茎伸
847和0.662
0.693
0.816。鳞茎
OAS-TL活性与硫含量整
【结论】大蒜生长过程中，氮、硫两元素间存在互
作关系。NR、ATPS等酶活性的提高增加了植株氮、硫同化能力，而GS则通过降低酶活性而促进氮的同化。在大蒜鳞茎膨大期前，氮、硫配施能够通过调控关键同化酶活性而影响氮、硫同化，进而影响植株生长；鳞茎
膨大阶段，可以通过单一施肥达到调控大蒜植株氮或硫含量的目的。关键词：大幕；氮：硫；酶活性；同化；Pearson相关系数
Nitrogenandsulfurinteractionincreasetheirassimilationandactivities
ofkeyenzymesingarlic
XU Jian'2,JIAKai'，ZHU Jun-fang',HUMei',WANGNa',GAO Jie
(I College of Forestry and Horticulture, Xinjiang Agricultural University, Urmuqi 830052, China; 2 Garden Science and
Technology College, Xinjiang Agricultural Vocational Technical College, Changji,Xinjiang 831100, China)
Abstract:【 Objectives ] Researching physiological effects of nitrogen and sulfur application on related enzymes e product quality.[ Methods】 A pot experiment using perlite and vermiculite as cultural substrate was performed. Two factors and three levels of the complete design were included. N was applied in levels of 5, 10 and 20 mmol/L and S in levels of 2, 4 and 8 mmol/L. Leaves and plants were sampled at the seedling stage, stem elongation stage, and bulb expansion preliminary and middle stages.【Results] The nitrogen content ofgarlic was on the rise, and achieved the highest level at the bulb expansion stage. The nitrate reduction (NR) activity and glutamine synthetase (GS) activity were increased at first and then dropped later, and the enzymatic activities were higher from the stem elongation stage to bulb expansion preliminary stage. The sulfur content of garlic showed a trend of rise and then steady. At the stem elongation stage, the ATP sulfurylase (ATPS) activity
收稿日期：2016-06-14
接受日期：2016-12-06
基金项目：新疆研究生科研创新项目（XJGRI2014075）；新疆维吾尔自治区园艺学重点学科基金项目资助。
作者简介：许建（1982—），男，江苏铜山人，博士，主要从事蕉菜裁培及生理研究。E-mail：xujay1982@163.com
*通信作者E-mail：13999803260@163.com
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