第34卷，第7期 2014年7月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol. 34 ,No. 7 -pp1742-1746
July，2014
DeterminationofComponentsofMixedColorSolutionsby
FluorescenceSpectroscopyCombined with
CalibrationAlgorithms
ZHU Chun'2,LIRun',QUELi-zhi',ZHUTuo",CHEN Guo-qing
1. School of Science, Jiangnan University , Wuxi 214122, China
2. School of Internet of Things Engineering , Jiangnan University , Wuxi 214122, China 3. College of Energy and Electrical Engineering , Hohai University , Nanjing210098, China
Abstract The three-dimensional spectra of mixed solutions of allure red , sunset yellow and brilliant blue were obtained . Then the three synthetic food colors were determined by parallel factor analysis (PARAFAC ) and alternating trilinear decomposition (ATLD ) algorithms , respectively . The component number of model is three by core-consistency diagnostic . The average recoveries of allure red , sunset yellow and brilliant blue obtained by PARAFAC were 98. 75% ±8. 9% , 97.22% ±2. 9% and 99. 00% ±2. 9% and those by ATLD algorithm were 99. 78% ±5. 9% , 92. 52% ±5. 5% and 97. 23% ±5. 8% , respectively . Results show that both of the al-gorithms can be used in direct and rapid determination of multi-components of mixtures . From further compar ison , the PARAFAC is more stable and advantageous
KeywordsSynthetic food colors ; Three-dimensional fluorescence spectroscopy ; PARA FAC algorithm : ATLD algorithm
中图分类号：0657.3
Introduction
文献标识码：A
DOI : 10. 3964 /i. issn. 1000-0593 (2014 )07-1742-05
Fluorescence spectroscopy has been widely used for its fastness , accuracy , high sensitivity and selectivity . Synthetic food colors are fluorophores C8.9] , which means that they can
In recent years , food safety accidents caused by the abuse of synthetic food colors happened occasionally such as *Sudan Red-yolk Duck Egg" and "Stained Steamed Bread" . It makes people draw extensive attention to the safety detection of syn-thetic food colors-3] , Recently + the detection methods of synthetic food colors are high performance liquid chromatog raphy + thin layer chromatography , spectrometer, capillary electrophoresis, ete-]. However, these methods always need time-consuming pretreatment and complicated measuring process , which makes it rather difficult to analyze the foods which contain complicated components . A new method which is more efficient should be studied .
Received : 2013-09-27 ; accepted : 2014-01-21
be analyzed by fluorescence spectroscopy . Nonetheless , the a e at o e re paradoa aio are saooa fluorophores in real food . The spectra of them may be over-lapped, which makes it difficult to directly detect the target component in mixed food colors solutions by fluorescence spectroscopy
Second order calibration algorithm has clear advantages in the simultaneous determination of target components in mixtures. For example, Wu Hailong's group realized the concentration determination of the chlorinated aromatic hydro -carbons and drug composition with ATLD algorithm [10-13] Raquel M . Callejon et al. and other groups applied the
Foundation item: The National Natural Science Foundation of China (61178032 , 61378037 ) and Jiangsu Province Science and Technology Sup-
port Program (Community Development ) (BE2011828 )
Biography : ZHU Chun , (1979), female , Jiangnan University , School of Science , lecturer . Main research area : Analysis and application of
fluorescence spectroscopy
e-mail : chunzhu1979@ hotmail .com
Corresponding author
e-mail : cgq2098@ 163. com