第33卷第3期 2013年9月
化学传感器 CHEMICALSENSORS
基于石墨烯-纳米金-丝素水凝胶的高灵敏
有机相酶电极检测呋喃丹
许绍鹏，董静2，艾仕云2
（1.山东省沾化县冯家镇第六小学，山东滨州256805）(2.山东农业大学化学与材料科学学院，山东泰安271018）
Vol.33, No. 3 Sept .2 013
摘要：采用改进的Hummers法制备了氧化石墨烯(GO),用微波辅助丝素还原法制备了丝素还原石墨烯还原的石墨烯-纳米金复合水凝胶包埋酪氨酸酶(Tyr)制备了新型的有机相酶电极(SF-GR-NanoAu-Tyr/ GCE),在纯氯仿中对呋哺丹进行了检测。结果表明，在有机相中，丝素水凝胶能较好的保持酪氨酸酶的生物活性，GR和NanoAu促进了电子在电极界面上以及水凝胶内部的电子传递，提高了酶电极的灵敏性。在没有另外添加水或者缓冲液的情况下，酪氨酸酶的抑制率与浓度范围为1.0×10-~1.0×10-2mol/L的呋哺丹呈线性关系，检测限为8.0×10-13mol/L。该有机相酶电极制备简单，检测快速，灵敏度高，适合于有机溶剂中微量农药的定量检测。
关键调：石墨烯；酪氨酸酶；纳米金；有机相酶电极；呋哺丹
Determinationofcarbofuranusingahighlysensitiveenzyme inhibitiontyrosinaseOPEEbasedongraphene-goldnanoparticles
silkfibroinhydrogel Xu Shao-peng', Dong Jing", Ai Shi-yun*"
(1. The Sixth Elementary School, Fengjia Town, Huazhan, Binzhou 256805, China)
(2. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, China
Abstract: Graphene oxide was prepared by the method of modified Hummers. Silkreducedgraphene (SFGR) was prepared using microwaveassisted silk fibroin reduction synthesis method. TEM, FTIR and UVvis were uti. lized to characterize SFGR. Then, a novel highly sensitive enzymatic inhibition organic phase enzyme electrode(OPEE) was fabricated by enwrapping tyrosinase in graphenegold nanoparticlessilk fibroin hydrogel. The SF hy-drogel provided a necessary amount of water and a biocompatible microenvironment around the enzyme molecule to stabilize its biological activity and effectively prevented its inactivation by inhibitors such as pesticides. Under op timized conditions, the inhibition percentage of carbofuran was proportional to its concentration in the range of 1.0 × 10-* to 1.0 × 10-12 mol/L with a detection limit of 8.0 × 10- mol/L. Thus, the highly sensitive OPEE is a promis. ing new tool for pesticide analysis in organic solvents.
Key words: graphene; tyrosinase; gold nanoparticles; carbofuran; organic phase enzyme electrode
基金项目：国家自然科学基金(No.21075078)和山东省自然科学基金(No.ZR2010BM005)资助项目净通讯联系人，E-mail:ashy@sdau.edu.cr