植物营养与肥料学报2013，19(5)：1083-1091 Joumal of Plant Nutrition and Fertilizer
doi:10.11674/zwyf.2013.0507
低磷胁迫对不同品种高梁苗期形态
及生理指标的影响
马建华1,2.4，王玉国1*，孙毅12.3·，尹美强'，牛常青4，杨艳君*
（1山西农业大学农学院，山西太谷030801；2山西省农业科学院生物技术研究中心，山西太原030031；3农业部黄土高原作物基因资源创制重点实验室，山西太原030031；
4山西省晋中学院生物科学与技术学院，山西晋中030600）
捕要：以14个高粱品种为材料，设置5个磷处理水平，对高架苗期的形态和生理指标进行了研究。结果表明，1）不同水平磷处理对高梁苗期各形态指标均有极显著影响，其中以0.25mmol/L作为低磷处理较适合；2)低磷胁迫对高架幼苗各形态指标均有不利影响，干重、植株地上部含磷量受低磷的影响较大，可作为耐低磷高粱品种的筛选指标；3)不同高桑晶种对低磷的耐性存在明显的基因型差异，聚类分析显示八月齐属相对耐低磷性强的品种，农 858属低磷敏感型品种；4）低磷下叶绿素和可溶性蛋白含量下降，丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化物酶(POD)活性升高，除可溶性蛋白外，叶绿素、MDA、SOD和POD活性均表现为耐低磷性强的相对变化率较小，耐低磷性弱的相对变化率较大。
关键词：高梁；低磷胁迫；植物形态；叶绿素；可溶性蛋白；丙二醛；超氧化物歧化酶；过氧化物酶
中图分类号：S514.01；Q945.78
文献标识码：A
文章编号：1008505X(2013)05-108309
Effects of low phosphorous stress on the morphologies and physiological
indices of different sorghum cultivars at seedling stage
MA Jian-hua'.2.4, WANG Yu-guo'* , SUN Yil.2, 3+ , YIN Mei-qiang', NIU Chang-qing , YANG Yan-jun(I College of Agriculture, Shanxi Agriculture Uninersity, Taigu, Shanxi 030801, China; 2 Biotechnolog) Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China; 3 Key Laboratory
of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China
4 College of Bioengineering, Jinzhong University, Jinzhong, Shanxi 030600, China)
Abstract: The effects of low P stress on the morphologies and physiological indices of sorghum were investigated with 14 sorghum cultivars at seedling stage. The results show, 1) Different P supply levels significantly affect the morphologies of sorghum seedlings (P <0. 01). P supply level of 0. 25 mmol/L was found to be the proper P level for screening P-tolerance cultivars; 2) Low P stress decreases the P content of shoot, and negatively influences morphological development of sorghum seedlings, especially on plant dry weight accumulation. Therefore shoot P content and above ground dry weight were recommonded as screening indices for low P-tolerance cultivars at sorghum seedling stage; 3) Different cultivars responded differently to low-P stress. Bayueqi was a low-P tolerance cultivar, while Nong858 was a low-P sensitive cultivar through cluster analysis; 4) P deficiency leads to low chlorophyll and soluble protein contents, high MDA content and high SOD and POD activities in sorgum seedlings. Except soluble protein, relative variations in the tested indices were small in low-P tolerance cultivars, high in low P sensitive cultivars.
Key words:sorghum;low phosphorous stress; morphology; chlorophyll;soluble protein; MAD;SOD;POD
收稿日期：201208-14
接受日期：20130326
基金项目：转基因生物新品种培育重大专项(2009ZX08003-017B）
作者简介：马建华（1977一），女，山西柳林人，博士研究生，讲师，主要从植物生物技术研究。E-mail；mjh778399@163.com
。通信作者E-mail;tgwygn@126.com;sunyi692003@yahoo.com.cn
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