植物营养与肥料学报2017,23(2):278-288 Journal of Plant Nutrition and Fertilizer
doi: 10.11674/zwyy.16103 http://www.plantnutrifert.org
低氮胁迫对不同耐低氮玉米品种苗期伤流液性状
及根系活力的影响
吴雅薇，李强，豆攀，孔凡磊，马晓君，程秋博，袁继超（农业部西南作物生理生态与耕作重点实验室/四川农业大学农学院，成都611130）
摘要：【目的】研究了低氮胁迫对不同耐低氮性玉米品种苗期生长、伤流性状及根系活力的影响，比较不同耐低氮性玉米品种对低氮胁迫响应的差异，以期为西南丘陵地区耐低氮玉米品种的选育提供理论依据。【方法】以两个耐低氮品种“正红311'、‘成单30和两个低氮敏感品种“先玉508'、‘三北2号，为试验材料进行水培试验，营养液设置4个氮水平：0、0.05、0.5、5mmol/L。测定了苗期株高等形态指标、干物质积累量、根系活力，计算了根冠比、活联吸收面积比、伤流液中可溶性蛋白等转运速度及氨基酸/硝态氮浓度的比值。【结果】
低氮胁迫下玉米苗期苗高、茎粗
、面表
和可溶性糖转运
著下降，但耐低氮品种各指标的降
高，可溶性蛋白和可溶性糖浓度随胁迫用
氮敏感品种，
而可落性蛋百浓度的增幅高于低氮缴感品
性蛋白和可溶性糖浓度显著高于低氨敏感品种，
上部干重、伤流强度，伤流液中可溶性蛋白
系总吸收面积和根系活跃吸收面积均显防流液中氨基酸/硝态氮浓度比值均显著升高的趋势。耐低氮品种根冠比增幅低于低
且低氮胁迫下耐低氮品种根系活力、伤流液中可溶
【结论】耐低氮品种在低氮胁迫下能够保持较高的根系活力，
进而促进了根系对营养物质的吸收和转运，提高了伤流液中各组分的浓度和转运速度，较好地维持了碳氮循
环，从而有效地协调了植株地上、地下部养分和物质的分配，控制了根冠比的增加，维持了地上部的生长。关键词：玉米；低氮胁迫；生长；伤流性状；根系活力
Effectof lownitrogenstressonbleedingsapcharactersandrootactivityof
maizecultivarswithdifferentlowNtolerance
WUYa-wei,LIQiang,DOU Pan,KONGFan-lei,MAXiao-jun,CHENG Qiu-bo,YUAN Ji-chao
(Key Laboratory of Crop Ecophysiology and Farming Systems in Southwest China, Ministry of Agriculture/College of Agronomy
Sichuan Agricultural University, Chengdu 611130, China)
Abstract:【Objectives]Insufficient nitrogen(N) supply impacts the growth and root activities of crops. Comparison of the bleeding characteristic responses of different low N tolerance maize cultivars will provide theoretical basis for breeding of low N tolerant maize cultivars.[Methods】Two low N tolerant cultivars(ZH311'and ‘CD30') and two low N sensitive cultivars (°XY508'and *SB2") were used as tested materials, and a hydroponic experiment with four N supply levels of 0, 0.05, 0.5 and 5 mmol/L was conducted. The plant height, dry matter accumulation, and root activity were investigated at seedling stage ofmaize.[Results] The stem diameters, plant heights, leaf areas, SPAD values, dry biomass of seedlings were decreased with the decreased N supply levels. The bleeding sap intensity, transport speeds of free amino acid, nitrate N, soluble protein and soluble sugar were declined, and the concentrations of free amino acid, nitrate N in bleeding, and the total absorbing areas, actively absorbing areas and root activities were decreased in all the four tested cultivars.
收稿日期：201603-17
接受日期：2016-09-08
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计划项目（2014NZ0040）；四川省玉米产业技术体系岗位专家项目资助
作者简介：吴雅薇（1993—），女，福建南平人，硕士，主要从事植物逆境生理方面的研究。E-mail：wyw6140@163.com
*通信作者E-mail：yuanjichao5@163.com
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