
植物营养与肥料学报2010,16(6):1366-1372 Plant Nutrition and Fertilizer Science
低磷胁迫对小麦代换系保护酶活性和丙二醛含量
的影响及染色体效应
郑金凤,董少鸣,李成璞3,白志英1*,李存东3:,毕常锐
(1河北农业大学生命科学学院,河北保定071000;2承德师专,河北承德067000:
3河北农业大学农学院,河北保定071001)
摘要:以中国春-Synthetie6x染色体代换系及其亲本为材料,通过测定孕穗期、开花期、灌浆期不同磷处理条件下叶片的保护酶活性及丙二醛含量,研究低磷胁迫对相关生理性状的影响及染色体效应。结果表明,低磷胁迫下,SOD
2A,5A、6A、7B、7D染色体上可能存在诱导POD活性增强的基因;5A、2D、5D、7D染色体上可能存在抑制MDA含量增高的基因。
关键词:低磷胁追;小麦代换系;保护酵;丙二醛;染色体效应
中图分类号:Q945.78
文献标识码:A
文章编号:1008505X(2010)061366-07
Effectsof phosphorusdeficiencystress onprotectiveenzymeactivities,
MDAcontentandchromosomeofwheatsubstitutionlines ZHENG Jin-feng" , DONC Shao-ming’, LI Cheng-pu", BAI Zhi-ying'', LI Cun-dong', BI Chang-rui
(I College of Life Science, Hebei Agricultural University, Baoding, Hebei 071000, China
2 Chengde Teachers' College for Nationalities, Chengde, Hebei 067000, China; 3 College of Agronomy, Hebei Agricultural Uninersity, Baoding, Hebei 071001, China)
Abstract: The effects of phosphorus deficiency stress on physiological characteristic and chromosome response in wheat was studied by determining protective enzyme activities and MDA content and locating the gene controlling protective enzyme activity and MDA content at diferent developing stages using wheat substitution lines between Chinese Spring (CS) and Synthetic 6x. The results showed that SOD and POD activities, and MDA content in creased under phosphorus deficiency stress. The genes promoting SOD activity might be located on 2A, 3B, 2D and 7D chromosome of Synthetic 6x; and the genes promoting POD activity might be located on 2A, 5A, 6A, 7B and 7D chromosome, while the genes inhibiting MDA content might be located on 5A, 2D, 5D and 7D chromosome of Synthetic6x.
Key word: P-deficiency; substitution lines; protective enzymes: MDA; chromosome effec
土壤缺磷属于典型的非生物逆境,是当今农业生产中限制作物生长与产量的主要因素之一(]。作物为了适应磷亏缺的生长环境,往往会在形态、生理、生化等方面发生一系列的变化(2)。许多研究表
收稿日期:2010-01-14
接受日期:2010-04-30
明,低磷胁迫与植物细胞保护酶活性和丙二醛含量密切相关(-)。
小麦全套品种间代换系是由21个成员组成的,其染色体数与成员数相等,每个成员与其受体品种
基金项目:"973"计划前期研究专项(2007CB116209);“十一五"国家粮食丰产科技工程项目(2006BAD02A08);河北省自然基金项目
(C2008000341)资助。
作者简介:郑金风(1982—),女,河北新河人,硕士研究生,主要从事植物资源利用与开发研究。E-mail;zhengjinfeng1982@126.com
● 通i讯作若 Tel; 03127521316, E-mail; zhiyingbai@ 126. com; E-mail; nxylod@ hebau, edu. cn 感谢John Innes Centre,Norwich Research Park提供试验材料。
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