植物营养与肥料学报2011,17(1)：110-116 Plant Nutrition and Fertilizer Science
大豆/玉米间作体系中接种AM真菌和根瘤菌
对氮素吸收的促进作用
李淑敏，武帆
（东北农业大学资源与环境学院，黑龙江哈尔滨150030）
摘要：利用大豆和玉米之间根系不同分隔方式的盆栽试验，研究了在玉米/大豆间作体系中接种大豆根瘤菌、AM 真菌Glomus mosseae和双接种对间作体系氮素吸收的促进作用。结果表明，双接种处理显著提高了大豆及与其间作玉米的生物量、氮含量，双接种大豆/玉米间作体系总吸氮量比单接AM菌根、根痛菌和不接种对照平均分别增加22.6%、24.0%和54.9%。大.促进了与其间作玉米对氮素的吸收作用，在接种AM真菌和双接种条件，间作玉米的AM真菌役染率提高，大豆根瘤数增加；接种AM真菌处理，不分隔和尼龙网分隔比完全分隔玉米吸氮量的净增加量是未接种对照的1.8、2.6借，双接种处理分别是对照的1.3和1.7倍。说明在间作体系中进行有效的根痛菌和AM真菌接种，发挥两者的协间作用对提高间作体系土壤养分利用效率，进一步提高间作体系的生产力有重要的意义。
关键调：大豆/玉米间作；大豆根瘤菌；吸氮量；AM真菌
中图分类号：S144.3
文献标识码：A
文章编号：1008505X(2011)010110-07
Nitrogen uptake facilitation in soybean/maize intercropping system
inoculatedwithrhizobiumandarbuscularmycorrhizalfungi
LI Shu-min, WU Fan
(Resource and Enwironmental College, Northeast Agriculural Uninersity, Harbin, Heilongjiang 150030, China)
Abstract: Pot experiments with different root barriers between maize and soybean were carried out to investigale the effects of rhizobium, arbuscular mycorrhizal fungi and interaction of rhizobium and arbuscular mycorrhizal fungi on nitrogen uptake in a soybean/maize intercropping system. The results show that under the dual inoculation with Glomus mosseae and soybean rhizobium SH212, biomasses, nitrogen contents of soybean and associated maize are significantly increased. On the average, the nitrogen uptakes by soybean/maize intercropping system inoculated with both microorganisms are increased 22.6%, 24.0% and 54.9% compared to those of the inoculated with arbuscular mycorrhizal fungi, rhizobium and control respectively. Soybean facilitates nitrogen uptake of the inter-cropping maize. Arbuscular mycorrhizal colonization of the intercropping maize and nodule numbers of the inter cropping soybean are increased significantly. These are the main reasons that net increases of nitrogen uptake by maize under the mesh barrier and no barrier treatments are 1. 8 and 2. 6 times than that of the control. And the net increases of N uptake by maize under the dual inoculation treatments are 1. 3 and 1. 7 times than that of the control respectively. These results suggest that the inoculation with both microorganisms in the intercropping system could improve soil nutrition efficiency and enhance productivity of the intercropping system by the synergy effects of rhizo bium and arbuscular mycorrhizal fungi.
Key wards: soybean/maize intercropping; soybean rhizobium; nitrogen uptake; arbuscular mycorrhizal fungi
收稿日期：2009-11-06
接受日期：2010-08-21
基金项目：国家白然科学基会（30670382,31071867）：第二批中国博士后科学基金特别资助（200902367）；黑龙江省教育厅科学技术研究项目
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作者简介：李收（1971一），女，黑龙江省宁安人，副教授，主要从事植物养分高效利用研究。
Tel;045155190753,E-mail; lishumin113@ yaboo.com.cn 万方数据