植物营养与肥料学报2013,19(5)：1224-1233 Jourmal of Plant Nutrition and Fertilizer
doi:10.11674/zwyf.2013.0523
生物有机肥对秦巴山区核桃园土壤微生物群落
和酶活性的影响
孙薇"，钱勋，付青霞"，胡婷，谷洁2，王小娟，高华1,2
（1西北农林科技大学资源环境学院，陕西杨凌712100；2陕西省循环农业工程技术研究中心，陕西杨凌712100）摘要：采用大田试验，探讨生物有机肥对秦巴山区核桃园土壤微生物群落结构及其代谢、酶活性和土壤理化性质的影响。结果表明，施人生物有机肥可改变土壤微生物群落结构，改变微生物对各大类碳源的利用，提高微生物碳源利用率、物种多样性和丰富度，常规施肥对土壤微生物群落结构影响不显著。生物有机肥可显著提高土壤蔗糖酶、酸性磷酸酶和脱氢酶活性，但对过氧化氢酶的影响不显著；常规施肥可提高土壤脲酶和脱氧酶活性，对其他酶活性没有显著影响。生物有机肥可显著提高土壤有机质，全量氮、磷、钾和速效磷含量，提高土壤pH值，使土填理
化性质得到改善；常规施肥也可在一定程度上提高土壤养分含量，但其效果不如生物有机肥。关键健调：生物有机肥；微生物群落；酶活性；土壤理化性质
文章编号：1008505X(2013)05-1224-10
中图分类号：S144；S154.3
文献标识码：A
Effects of bio-organicfertilizer on soil microbial community and enzymes
activitiesinwalnutorchardsof theQinling-BashanRegion SUN Weil, QIAN Xun', FU Qing-xia', HU Ting", GU Jie'2, WANG Xiao-juan', GAO Hua'2(1 Collage of Resource and Enrironment, Northaest A&F Unirersity, Yangling, Shaanxi 712100, China
2 Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Prorince, Yangling, Shaanxi 712100, China) Abstract: A field experiment was conducted in Qinling-Bashan Region of Shaanxi province to investigate the influence of different fertilization treatments on soil microbial community, enzymes activities and soil nutrients in walnut orchards. There were three treatments, control ( CK), conventional fertilizer treatment (TI) and bio-organic fertilizer treatment (T2). The effect of bio-organic fertilizer addition on microbial community metabolic profiles was analyzed with the Biolog (ECO Microplate) method. The results show that the average well color development ( AWCD) , Shannon index and richness index (S) of microbial community of the T2 treatment are all significantly higher than those of the T1 treatment. Utilization of six carbon source types by soil microbial community shows that the soil microbial community of the bio-organic fertilizer treatment enhances consumption of all the six carbon source types, which indicatles the soil microbial community function is changed to some extent. Principal components analysis demonstrates that there are larger differences on utilization of carbon substrates by soil microbial community between the T2 and CK treatment and between the T1 and control, which indicates that the function of soil microbial community is modified by fertilization regimes. The soil sucrase, acidic phosphatase and dehydrogenase activities are improved significantly, and soil urease activity is changed significantly after the application of bio-organic fertilizer in comparison with the control, while soil catalase activities are similar between the two treatments. The contents of total soil nitrogen, total phosphorus, total potassium and available phosphorus of the T2 treatment are significantly higher than those of the CK. In addition, soil pH is increased in the T2 treatment, and decreased in the TI treatment in comparison with CK treatment. It is concluded that application of
接受日期：20130429
收稿日期：2013-01-16
基金项目：国家林业局科技推广项目（[2010]39）；国家自然科学基金（40871119，41171203）；农业部"948"项目（2010-220）资助。作者简介：孙薇（1987-—），女，山东淄博人，硕士研究生，主要从事环境微生物方面的研究。E-mail：sunwei20090@163.com
*通信作者E-mailgaohua163.com
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