植物营养与肥料学报2017,23(2)：444-450 Journal of Plant Nutrition and Fertilizer
doi: 10.11674/zwyf.16283 http://www.plantnutrifert.org
不同价态硒缓解小油菜镉胁迫的生理机制
周健，郝苗，刘永红，付庆灵，朱俊，胡红青
（华中农业大学资源与环境学院，农业部长江中下游耕地保育重点实验室，武汉430070）
摘要：【目的】硒有利于提高植物对环境胁迫的抗性，缓解植物受到的非生物胁追。研究不同价态硒对小油菜缓解重金属镉胁迫的影响，可为治理农田土壤重金属镉污染提供参考。【方法】采用盆裁试验，选取三种价态硒，分别为硒代甲硫氨酸Se(-2)、亚酸钠Se(+4)和硒酸钠Se(+6)，硒浓度均为1mg/kg，设置镉胁迫浓度0、低浓度辐(2mg/kg)和高浓度镉（5mg/kg)，共计12个处理，采用原子吸收光谱法测定植物的镉含量，分析调查了对小油菜生长及生理特性的影响。【结果】未加外源镉时，与对照(CK)相比，各价态Se均能显著促进小油菜生长，且Se(-2)处理对小油菜生物量、株高、根长的促进效果优于无机硒Se(+4)和Se(+6)处理；低
浓度胁迫下，与对照（CK）相比，经Se
而Se(+6)处理却增加了25% 地上部辐含量降幅可达139
生品
当存在镉胁迫时，施用Se(-2)和Se(+4
(2)和
4)处理后，小油菜地上部含量分别降低13%和5%，
经Se(-2)和Se(+4)处理后，小油菜根部及
小油菜根部及地上部镉含量分别增加了38%和17%。高植物体内超氧化物歧化酶（SOD)、过氧化物酶(POD）
和过氧化氢酶(CAT)等酶的活性，
增加小油菜叶片内抗坏血酸（AsA)和还原型谷胱甘肽（GSH)的含量，但
【结论】在胁迫下，Se(-2)在
Se(+6)降低了其抗氧化酶的活性，减少了小油菜叶片中AsA、GSH的含量。
促进小油菜生长、抑制镉在小油菜体内的积累及增强小油菜生理特性方面的作用均优于Se(+4)和Se(+6)。因
此，Se(-2)处理最能有效缓解小油菜辐胁迫，Se(+4)次之，Se(+6)却增加了镉对小油菜的胁迫作用。关键词：小油菜；硒；辐胁迫
Physiological mechanismof differentvalencesseleniuminrelieving
cadmiumstressofrape
ZHOU Jian, HAO Miao, LIU Yong-hong, FU Qing-ling, ZHU Jun, HU Hong-qing
[ College of Resources and Environment, Huazhong Agriculural University, Key Laboratory of Arable Land Conservation (Middle
and Lower Reaches of Yangtze River), Ministry of Agriculture, Wuhan 430070, China ]
Abstract:[ Objectives 】 Selenium contributes to improve resistance ability of plant under environmental stress, and can alleviate the abiotic stresses. Effect of different valences selenium on reducing Cd stress in rapeseed plants was investigated, which could provide a practical reference for cadmium polluted soils. [ Methods ] A pot experiment was carried out using three valences of selenium, they were Selenomethionine [Se (2)], selenium[Se (+4)] and sodium selenite [Se (+6)], the concentration of selenium was 1 mg/kg soil. Three stress levels of cadmium were designed as O, 2 and 5 mg/kg.The cadmium in plants was measured by atomic absorption spectroscopy, and the growth and physiological index of rapeseed were determined.[Results] Without Cd stress, compare to the CKe, all the tested Se could significantly promote the rape growth, and the Se (2) and Se(+4) were superior than Se (+6) in the promoting the biomass, plant height, root length of rape. Under stress of Cd 2 mg/kg, the Se (2) and Se (+4) significantly reduced the Cd accumulation in shoots of rapeseed by 13% and 5%, while Se (+6) increased that in roots by 25%. Under stress of Cd 5 mg/kg, and compared to the CK, the Se (2) and Se(+4) reduced the Cd contents in the rapeseed roots and shoots by 13%~41%, while the Se
收稿日期：2016-07-18
接受日期：2016-09-28
基金项目：国家科技支撑计划（2015BAD05B02）资助。
作者简介：周健（1991一），女，广西玉林人，硕土研究生，主要从事重金属污染修复研究。
E-mail:zhoujian0209@webmail.hzau.edu.cn。·通信作者 E-mail:hqhu@mail.hzau.edu.cn
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