
植物营养与肥料学报2016,22(2):511-517 Journal of Plant Nutrition and Fertilizer
doi10.11674/zwyf.14513 http://www.plantnutrifert.org
不同生长时期干旱胁迫甘薯根系生长及
荧光生理的特性比较李长志,李欢,刘庆,史衍玺*
(青岛农业大学,山东青岛266109)
摘要:【目的】干旱胁迫是我国甘薯生产所面临的重要间题,本试验旨在为研究甘薯的抗早生理机制,明确甘薯水分临界期,减轻干早影响和优化甘薯生产管理提供科学依据。【方法】利用人工旱棚,设置甘薯正常供水与前期、中期和后期(即定裁后15d、55d、95d)干早胁迫四个处理(田间持水量的8%~10%,每次胁迫持续15天)。用 Epsonv700扫描仪调查了不同处理三个不同时期根系发育,用M-PEA(Hansatech,英国)测定了叶片荧光生理参数。【结果】前期和中期干旱均显著降低了甘薯地上和地下部生物量(P<0.05),而后期干旱影响较小,其规律表现为前期>中期>后期。其中,前期于卓甘薯总生物量减少约50%;中期于卓造成地上和地下部分别减少38.4%和
31.1%;后期干旱地上和地下部减少均约10%左
胁迫均显著影响甘薯根系发育(P<0.05)。
与正常供水相比,前期干早胁迫总根长、总表面积和总体积分别减少49.5%、55.7%和43.2%,中期干早胁迫分别 6 和中期胁迫处理测定值差异显著,叶绿素荧光动力学曲线发生明显变化,后期未达显著性水平。与对照相比,前期和中期的PSⅡ综合荧光参数指标Fm、Fv/Fm、PI(ABS)分别减少了36.4%、15.6%、44.3%和14.7%、3.8%、 22.6%;指示反应中心活性的参数sEo分别减少7.7%、3.4%,而反映电子传递速率的参数Vi、dV/dto分别增加了33.1%、32.1%和19.2%、17.1%,这些荧光参数的变化表明,干早胁迫导致PSⅡ结构受损,反应中心受到伤害,光能转化效率降低,电子传递受阻。【结论】甘薯水分临界期处于前中期,实际中应特别加强甘薯前期的水分供应。
关键词:甘薯;干旱胁迫;根系发育;叶绿素荧光参数
中图分类号:S531
文献标识码:A
文献编号:1008505X(2016)020511-07
Comparison of root development and fluorescent physiological characteristics
of sweetpotato exposureto drought stress in differentgrowth stages
LI Chang-zhi, LI Huan, LIU Qing, SHI Yan-xi'
(QingdaoAgriculturalUninersity,Shandong,Qingdao266109,China)
Abstract: 【Objectives]To clearity the water critical period of sweet potato will provide scientific basis to find effective way against drought and optimization of production and management of sweet potato. [Methods] A pot experiment was conducted inside an artificial drought shed. Three drought stress (8%10% field water capacity, stress lasted 15 days for each treatment) was designed at early, middle and late stages of sweet potato (15, 55 and 95 days since transplanted).The root development was measured using Epson v700 Scannor, and the fluorescence physiological parameters using M-PEA( Hansatech , Britain) method. [Results ] The shoot and root biomass of sweet potato were significantly reduced when exposure to drought stress at both the early and middle growth stages (P < O. O5), but not significant at late stage drought stress, and the reducing effect was in order of early stage > middle stage >late stage. Compared with the control, the shoot and root biomass were reduced almost in half in the early
收稿日期:2014-11-14
接受日期:2015-0420
基金项目:现代农业产业技术体系建设专项资金(CARS-11-B-14):青岛市青年专项基金(14-2-4-117-jch);青岛农业大学博士基金
(6631314)资助。
作者简介:李长志(1990—),男,山东垦利人,硕士研究生,主要从事植物营养研究。E-mail:9731397@qq-com
*通信作者E-mail:yanxiy@126.com