
植物营养与肥料学报2014,20(4):870-876 Journal of Plant Nutrition and Fertilizer
doi: 10. 11674/zwyf. 2014. 0408
根际pH对玉米利用磷酸单酯和双酯盐的影响
王悦,符力,冯固*
(中国农业大学资源与环境学院,北京100193)
摘要:【目的】土壤有机磷在土壤全磷中占有很大比重,是植物潜在的有效磷源,但必须通过磷酸酶的水解作用释放出无机磷才能被植物利用。土壤中有机磷的主要形式为磷酸单酯和磷酸双酯。本研究中,我们探讨了无菌条件下不同形态的氮源引起的根际H变化如何影响植物对这两种有机磷的活化利用。【方法1采用琼脂无菌培养体系种植玉米,向玉米植株供应两种形态的氮源和磷源,氮源为硝态氮和铵态氮,磷源为植酸钙和卵磷脂,植酸钙属于磷酸单酯盐,卵磷脂属于磷酸双酯盐。不同的供氨形态会导致根际pH变化,进而研究根际H变化对磷酸单酯盐和磷酸双酯盐的活化利用所产生的影响。【结果】当给玉米供应铵态氮时,根际pH从5.5降至4.0;供应硝态氮
时,根际pH升至6.6
pH 6. 0.
测定玉米根际的琼脂中根系分泌的磷酸单酯酶和磷酸双酯酶活性发现,磷酸单酯酶活性在
之最高,薛酸双留爵活性在D
相对于铵态氮。酶活性和底物有
际pH值降低Ⅲ开高
大,它的质激有效性要高两种磷酸酶活性较
联物有液中的壤固有或
无论以植酸钙还是卵磷脂为有机磷源,
水解过程由磷酸钙的溶解度随根
时,根际pH值降低,玉
可时,任供应教态规系件下,箱校
酯酶活性较低,由于植酸钙的溶解度较生供应确念数时,确股钙溶解度微小而
磷酸酶活性和有机磷
溶解于水溶
范围股化,,
无疑对土
献的,应该在北方土壤养分管理中应加以考虑
关键调:无菌培养;有机磷;磷酸单酯酶;磷酸双酯酶;根际pH
中图分类号:S513;S945.12
文献标识码:A
文章编号:1008-505X(2014)04-087007
EffectsofrhizospherepHonutilizationofPinphytinandlecithinbymaize
WANG Yue, FU Li, FENG Gu
(College of Resources and Enzironmental Sciences, China Agricaultaural Uniwersity, Beijing 100193, China)
Abstract:[ Objectives]Soil organic phosphorus is accounted for a large proportion in total soil phosphorus, and is a kind of potential available phosphorus. Hydrolyzing by phosphatase is the only way obtaining phosphorus for the plants. There are mainly two forms of organic phosphorus in the soil, one is phosphomonoester, and the other one is phosphodiester. An axenic agar cultivation in Petri dish was conducted to study effects of pH on phosphomonoesterase or phosphodiesterase utilization in maize rhizosphere. [ Methods] The method of an axenic agar in Petri dish was used in this experiment. Two kinds of nitrogen forms (NO, and NH+ ) and two kinds of organic phosphorus forms (phytin: phosphomonoester and lecithin : phosphodiester) were supplied in the maize rhizosphere. Different nitrogen forms will lead to the pH changes in rhizosphere, which can be used to evaluate the effects of pH on phosphomonoesterase or phosphodiesterase utilization in maize rhizosphere. [Results ] When the NH, form was supplied, rhizosphere pH was decreased from 5. 5 to 4. 0, while nitrogen was supplied in the NO,
收稿日期:2013-08-10
接受日期:2013-12-12
基金项目:高等学校博士学科点专项科研基金课题(20120008130001);北京市自然基金课题(6132019)资助。
作者简介:王悦(1989—),女,辽宁朝阳人,硕士研究生,主要从事磷调控机理方面研究。E-mail:15652739905@163.com
*通信作者 Tel:010 62733885, E-mail; fenggu@ cau. edu.c