第31卷第1期 2011年2月
广东海洋大学学报
Journal of Guangdong Ocean University
Vol.31 No.1 Feb.2011
甲状腺素对斜带石斑鱼卵黄囊期仔鱼生长发育的影响
唐啸尘1，周歧存2，丁1，张海发3，王云新3
（1.广东海洋大学水产学院，广东湛江524025；2.宁波大学生命科学与生物工程学院浙江宁波510275
3.广东省大亚湾水产试验中心，广东惠州516081）
摘要：用4种不同浓度的甲状腺素（T4）分别浸泡斜带石斑鱼卵黄囊期仔鱼，研究T4对存鱼发育、生长和存活的影响。结果表明，在孵化后24h内，T4对存鱼发育的促进作用不明显；在孵化24h后，T4对鱼消化道的发育有促进作用。在孵化后72h，0.01mg·L-1T4处理组存鱼小肠管径、直肠管径、全长与对照组相比差异均不明显；0.10mg-L-和1.00mg-L-1T4处理组仔鱼小肠和直肠管径显著比对照组大（P<0.01），1.00 mg-L-T4处理组存鱼全长显著比对照组短（P<0.01），而0.10mg·L处理组存鱼全长与对照组相比无明显差异（P>0.05)。在孵化后第8天，0.10mg·L-T4处理组仔鱼存活率明显比对照组高（P<0.01).而 0.01mg-L-"T4组和1.00mg-LT4组与对照组之间存活率差异不明显（P>0.05)。结果提示0.10mg·L-T4
处理能促进斜带石斑鱼卵黄囊期存鱼的消化道发育并提高其存活率。关键词：斜带石斑鱼；卵黄囊仔鱼；消化道发育；生长；甲状腺素
中图分类号：Q959.483.4
文献标志码：A
文章编号：1673-9159(2011)01-0018-05
Effectof Thyroxine onGrowth and Development of
EpinepheluscoioidesYolk-sacLarvae
TANG Xiao-chen', ZHOU Qi-cun", DING Yu', ZHANG Hai-fa', WANG Yun-xing
(1.Fisheries College of Guangdong Ocean University, Zhanjiang 524025, China: 2.Faculry of Life Science and Biotechnology of Ningbo Universiry, Ningbo 315211, China; 3. Guangdong
Daya BayFisheries Development Center,Huizhou 516081,China)
Abstract: The effect of four doses of thyroxine (T4) on orange-spotted grouper (Epinephelus coioides) yolk-sac larvae was studied. The effect of T4 on larval development was not obvious within 24 hours post-hatching (hph). However, the advancement in development of gut was induced by T4 treatment after 24 hph. The differences between the control and 0.01 mg · L-1 T4 groups were not observed at 72 hph. Compared with the control, the diameter of larval intestine and rectum in both 0.10 mg · L-' and 1.00 mg L-' T4 groups were obviously longer at 72 hph ( P < 0.01 ) , however, the larval total length in 1.00 mg - L-l T4 groups was obviously shorter ( p 0.01 ), while the larval total length were not significantly affected by 0.10 mg · L-' T4 treatment ( p > 0.05 ) . The rate of survival in 0.10 mg - L-I T4 groups was obviously higher than that in the control by 8 days post-hatching ( p < 0.o1 ) , while that in either 0.01 mg - L- groups or 1.00 mg - L-T4 groups was not( P > 0.05 ). The results indicated that 0.10 mg · L- T4 treatment could promote Epinephelus coioides alimentary development and improve the rate of survival.
Key words: Epinephelus coioides; yolk-sac larvae; alimentary development; growth; thyroxine 收稿日期：2010-11-29
基金项目：广东省科技计划项目(2010B020308008)：广东海洋大学博土启动项目（0812269）
第一作者：唐哺尘（1969一），博士，副教授，研究方向为鱼类生理学。Email：txe816e163.con 万方数据