第7卷第4期 2016年4月
食品安全质量检测学报 Journal ofFood Safety andQuality
农产品中转基因检测抽样研究-第2部分：
从分析样品到试料
徐静，邵筠乔，赵禹，张琳，汪霖，郑江，曹际娟
(辽宁出入境检验检疫局，大连116001)
Vol. 7 No. 4 Apr.，2016
摘要：目的针对农产品中转基因检测抽样过程，研究从分析样品到试料的抽样和分析过程各因素对结果准确性的影响。方法取非转基因玉米为基体，添加12个品系转基因玉米制作标准分析样品，按系统抽样方法抽取试样，以随机顺序分别测试添加的转基因含量，计算其单次相对标准差，作为分析样品的总相对标准差(单次分析)的度量。结果影响标准差的参数包括含量axs、粒度das、试料量Mrp、构成试料的份样数nm等，降低总分析相对标准差的主要途径是减小分析样品的粒度、增加试料量和增加试料的份样数。结论得出从分
析样品到试料过程中，粒度、试料量和重复次数的建议值，为该部分抽样方法提供了参考。关键词：转基因：抽样；农产品
Samplingforgeneticallymodified organism contentanalysis in agricultural
products-part 2: process from analytical sample to test portions
XU Jing, SHAO Yun-Qiao, ZHAO Yu, ZHANG Lin, WANG Lin, ZHENG Jiang, CAO Ji-Juan
(LiaoningEntry-Exit Inspection and QuarantineBureau,Dalian 116001,China)
ABSTRACT: ObjeetiveTo study the effects of the process from analytical sample to test portions on the accuracy of results during sampling of GMO content analysis in agricultural products. MethodsTaking non-GMO maize as matrix, 12 strains of GM maize were added to produce standard analytical samples. According to the systematic sampling method, GMO content in these samples were tested in random order and the relative standard deviation(RSD) was calculated as the mcasure to total RSD (single analysis). Results Factors affecting RSD included GMO content aas, particle size das, test portion mass Mrp and the number of increments composing the test portion n The main ways to reduce total RSD were to reduce the particle size, increase the mass of test portion and the number of increments.ConclusionAccording to the experimental results,the recommended value including the particle size, mass of test portion and the number of duplicate analysis are given for the process from analytical sample to test portions.
KEY WORDS: genetically modified organisms; sampling; agricultural products
基金项目：基金项目转基因生物新品种培育重大专项项目、转基因生物抽制样和精准检测技术项目(2014ZX08012-001)）
Fund: Supported by the Genetically Modified Organisms Breeding Major Projects and Genetically Modified Organisms Sample Preparatior and Accurate Detection Technology Projects(2014ZX08012-001)
*通讯作者：曹际娟，博士，研究员，主要研究方向为食品安全。E-mail:cij0909@163.com
*Cerresponding author: CAO Ji-Juan, Ph.D., Professor, Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China. E-mail:
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