·1492.
张延平，等
规方法省时、省力。同时也具有高通量的能力，可广泛应用于犬和猴病原菌的快速检测。
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鱼藤酮致帕金森病大鼠黑质中Smac和Bcl-2的表达及意义张延平！，李彦改"，徐晓臣"，王英态"，李印杰
(编校：徐萌）
Changes of expression of apoptosis -related proteins Smac and Bcl -2 in Parkinsons diseaseratinducedbyRotenone
Zhang Yanping",Li Yangai',Xu Xiaochen', Wang Yingjie',Li Yinjie?
'Department of Neurology ,the First Hospital of Handan City,Hebei Handan 056004, China;'Department of Neurology, Handan Electric Power Hospital,Hebei Handan 056002, China.
[Abstract] Objective: To study the changes of expression of apoptosis related proteins Smac and Bcl 2 in the midbrain substantia nigra in Parkinsons disease rat induced by Rotenone, Methods : Witstar rats were randomly divid-ed into control group and experimental group. Control group 10 rats were treated by subcutaneously injection of sun-flower oil 1ml/kg,25 in experimental group were divided into A,B,C three groups,with 3. 0(5) ,2. 0(10) and 1. 0(10) mg/( kg · d) (subcutaneous injection of Rotenone dissolved in sunflower oil, shake evenly mixed 4C stored a-way from light). Results : TEM observated results , neuronal cells in experimental group shrinkage Rotenone treatment, cytoplasmic dense,the nuclear chromatin,nuclear fragmentation and some cells,cytoplasmic buds abscission,and for-mation of apoptotic bodies, And with the increase of the dose of Rotenone,apoptotie body formation was more obvious.
【收稿日期】【基金项目】【作者单位】
【作者简介】
2014 -10 17
邯郸市科技局资助项目（编号：1428108049-2） 1邯郸市第一医院神经内科，河北
邯郸
056004
3邯郸电力医院神经内科，河北邯郸056002
张延平（1974-），女，河北唐县人，硕士研究生，主治医师，主要从事帕金森病的治疗与防治研究。E-mailxhangyanping3212@ 163.com